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鉴定 8 个多态性微卫星位点用于巨石斑鱼(Epinephelus lanceolatus Bloch)。

Characterization of eight polymorphic microsatellite loci for the giant grouper (Epinephelus lanceolatus Bloch).

机构信息

Department of Oceanography, Xiamen University, Xiamen 361005, China.

出版信息

Mol Ecol Resour. 2008 Jul;8(4):805-7. doi: 10.1111/j.1755-0998.2007.02070.x.

DOI:10.1111/j.1755-0998.2007.02070.x
PMID:21585897
Abstract

Eight polymorphic microsatellite loci were isolated and characterized using a small insert genomic DNA library for the giant grouper (Epinephelus lanceolatus Bloch, 1790), a commercially valuable marine fish in tropical waters. They showed polymorphism information content ranging from 0.177 to 0.775, allele numbers ranging from two to 10, effective allele numbers ranging from 1.227 to 5.012, and observed and expected heterozygosities from 0.2 to 0.733 and from 0.185 to 0.801, respectively, which we anticipate will be useful for population genetic studies of the giant grouper.

摘要

利用小插入基因组 DNA 文库,我们分离并鉴定了 8 个多态性微卫星基因座,这些基因座用于研究商业价值很高的热带海水养殖鱼类——斜带石斑鱼(Epinephelus lanceolatus Bloch, 1790)。这些微卫星基因座的多态信息含量范围为 0.177 至 0.775,等位基因数范围为 2 至 10,有效等位基因数范围为 1.227 至 5.012,观测杂合度和期望杂合度范围分别为 0.2 至 0.733 和 0.185 至 0.801。这些结果有望为斜带石斑鱼的种群遗传研究提供有用的信息。

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