分析超速离心法中静止转子温度的测量。
Measurement of the temperature of the resting rotor in analytical ultracentrifugation.
作者信息
Ghirlando Rodolfo, Zhao Huaying, Balbo Andrea, Piszczek Grzegorz, Curth Ute, Brautigam Chad A, Schuck Peter
机构信息
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
Anal Biochem. 2014 Aug 1;458:37-9. doi: 10.1016/j.ab.2014.04.029. Epub 2014 May 4.
Abstract
Accurate measurements of rotor temperature are critical for the interpretation of hydrodynamic parameters in analytical ultracentrifugation. We have recently developed methods for a more accurate determination of the temperature of a spinning rotor using iButton temperature loggers. Here we report that the temperature measured with the iButton on the counterbalance of a resting rotor, following thermal equilibration under high vacuum, closely corresponded to the temperature of the spinning rotor with a precision better than 0.2°C. This strategy offers an inexpensive and straightforward approach to monitor the accuracy of the temperature calibration and determine corrective temperature offsets.
摘要
准确测量转子温度对于解析超速离心法中流体动力学参数至关重要。我们最近开发了一些方法，可使用iButton温度记录器更准确地测定旋转转子的温度。在此我们报告，在高真空下热平衡后，用iButton测量静止转子平衡锤上的温度，与旋转转子的温度紧密对应，精度优于0.2°C。该策略提供了一种廉价且直接的方法来监测温度校准的准确性并确定校正温度偏差。
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