Instituto de Neuroetología, Universidad Veracruzana, Avenida Dr. Luis Castelazo s/n, Colonia Industrial Ánimas, 91001 Xalapa, VER, Mexico ; Facultad de Química Farmacéutica Biológica, Universidad Veracruzana, 91190 Xalapa, VER, Mexico.
Facultad de Química Farmacéutica Biológica, Universidad Veracruzana, 91190 Xalapa, VER, Mexico ; Programa de Tutoría para la Investigación, Universidad Veracruzana, 91001 Xalapa, VER, Mexico.
Biomed Res Int. 2014;2014:938060. doi: 10.1155/2014/938060. Epub 2014 Apr 1.
In previous studies, the anxiolytic-like effects of Montanoa tomentosa and Montanoa frutescens were reported in male rats, but the potential anxiolytic-like effects of Montanoa plants during the different phases of the ovarian cycle in rats remain to be explored. The anxiolytic-like effects of the aqueous crude extracts of M. frutescens (25 and 50 mg/kg) and M. grandiflora (25 and 50 mg/kg) in the elevated plus maze were investigated in Wistar rats during the estrous cycle and compared with 2 mg/kg diazepam as a reference anxiolytic drug. To investigate any motor effect (i.e., hyperactivity, no changes, or hypoactivity) associated with the treatments, the rats were evaluated in the open field test. The M. frutescens (25 and 50 mg/kg) and M. grandiflora (50 mg/kg) extracts exerted anxiolytic-like effects during the metestrus-diestrus phase, similar to diazepam, without disrupting spontaneous motor activity. No significant effects of the extracts were detected in either behavioral test during the proestrus-estrus phase, whereas diazepam produced motor hypoactivity in the open field test. These results indicate that the M. frutescens and M. grandiflora extracts possess anxiolytic-like effects that depend on the ovarian cycle phase, supporting the Mexican ancient medicinal use of these plants to ameliorate anxiety disorders.
在之前的研究中，已经报道了蒙塔诺亚属植物（Montanoa tomentosa 和 Montanoa frutescens）对雄性大鼠具有抗焦虑样作用，但蒙塔诺亚属植物在大鼠卵巢周期不同阶段的潜在抗焦虑样作用仍有待探索。本研究旨在探究蒙塔诺亚属植物水提粗提物（M. frutescens[25 和 50mg/kg]和 M. grandiflora[25 和 50mg/kg]）在发情周期的雌性大鼠高架十字迷宫中的抗焦虑样作用，并与 2mg/kg 地西泮作为参考抗焦虑药物进行比较。为了探究与处理相关的任何运动效应（即过度活跃、无变化或活动减少），将大鼠在旷场试验中进行评估。在动情前期-动情后期，M. frutescens（25 和 50mg/kg）和 M. grandiflora（50mg/kg）提取物表现出抗焦虑样作用，与地西泮相似，而不破坏自发运动活动。在发情前期-动情期，提取物在两项行为测试中均未产生显著作用，而地西泮在旷场试验中产生运动活动减少。这些结果表明，M. frutescens 和 M. grandiflora 提取物具有依赖卵巢周期阶段的抗焦虑样作用，支持墨西哥古代医学使用这些植物来改善焦虑障碍。
