Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing.
作者信息
Bianco B, Diaspro A
机构信息
Department of Biophysical and Electronic Engineering, Genova, Italy.
出版信息
Cell Biophys. 1989 Dec;15(3):189-99. doi: 10.1007/BF02989683.
PMID:2480180
Abstract
The properties of an optical microscope are analyzed and analytically evaluated with a simple and effective model in order to understand the true meaning, limitations, and real capabilities of a defocusing technique. Major emphasis is given to the applications related to microscopic objects of biological interest using fluorescence and absorption light microscopy. A procedure for three-dimensional viewing is analyzed and discussed.
摘要
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