Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, New Zealand.
Microb Ecol. 2014 Oct;68(3):542-55. doi: 10.1007/s00248-014-0427-0. Epub 2014 May 7.
The South African invasive legume Dipogon lignosus (Phaseoleae) produces nodules with both determinate and indeterminate characteristics in New Zealand (NZ) soils. Ten bacterial isolates produced functional nodules on D. lignosus. The 16S ribosomal RNA (rRNA) gene sequences identified one isolate as Bradyrhizobium sp., one isolate as Rhizobium sp. and eight isolates as Burkholderia sp. The Bradyrhizobium sp. and Rhizobium sp. 16S rRNA sequences were identical to those of strains previously isolated from crop plants and may have originated from inocula used on crops. Both 16S rRNA and DNA recombinase A (recA) gene sequences placed the eight Burkholderia isolates separate from previously described Burkholderia rhizobial species. However, the isolates showed a very close relationship to Burkholderia rhizobial strains isolated from South African plants with respect to their nitrogenase iron protein (nifH), N-acyltransferase nodulation protein A (nodA) and N-acetylglucosaminyl transferase nodulation protein C (nodC) gene sequences. Gene sequences and enterobacterial repetitive intergenic consensus (ERIC) PCR and repetitive element palindromic PCR (rep-PCR) banding patterns indicated that the eight Burkholderia isolates separated into five clones of one strain and three of another. One strain was tested and shown to produce functional nodules on a range of South African plants previously reported to be nodulated by Burkholderia tuberum STM678(T) which was isolated from the Cape Region. Thus, evidence is strong that the Burkholderia strains isolated here originated in South Africa and were somehow transported with the plants from their native habitat to NZ. It is possible that the strains are of a new species capable of nodulating legumes.
南非入侵豆科植物 Dipogon lignosus（菜豆族）在新西兰（NZ）土壤中产生具有确定性和不确定性特征的根瘤。十种细菌分离株在 D. lignosus 上产生功能性根瘤。16S 核糖体 RNA（rRNA）基因序列将一种分离株鉴定为 Bradyrhizobium sp.，一种分离株鉴定为 Rhizobium sp.，八种分离株鉴定为 Burkholderia sp.。Bradyrhizobium sp.和 Rhizobium sp.16S rRNA 序列与以前从作物植物中分离出的菌株的序列相同，可能来自于作物上使用的接种剂。16S rRNA 和 DNA 重组酶 A（recA）基因序列将这八种 Burkholderia 分离株与以前描述的 Burkholderia 根瘤菌物种分开。然而，这些分离株与从南非植物中分离出的 Burkholderia 根瘤菌菌株在氮酶铁蛋白（nifH）、N-酰基转移酶结瘤蛋白 A（nodA）和 N-乙酰氨基葡萄糖基转移酶结瘤蛋白 C（nodC）基因序列方面表现出非常密切的关系。基因序列和肠杆菌重复基因间一致（ERIC）PCR 和重复元件回文 PCR（rep-PCR）带型表明，这八种 Burkholderia 分离株分为一个菌株的五个克隆和另一个菌株的三个克隆。一种菌株进行了测试，并证明能够在一系列南非植物上产生功能性根瘤，这些植物以前被报道为 Burkholderia tuberum STM678(T) 所结瘤，该菌株从开普地区分离得到。因此，有强有力的证据表明，这里分离到的 Burkholderia 菌株起源于南非，并以某种方式与植物一起从其原生境运输到新西兰。这些菌株有可能是一种能够结瘤豆科植物的新物种。
