School of Biological Sciences, University of Ulsan, Ulsan, Korea.
Plant Biol (Stuttg). 2015 Jan;17(1):34-40. doi: 10.1111/plb.12187. Epub 2014 May 6.
We investigated the relationship between the blue light receptor phototropin 1 (phot1) and lateral root growth in Arabidopsis thaliana seedlings. Fluorescence and confocal microscopy images, as well as PHOT1 mRNA expression studies provide evidence that it is highly expressed in the elongation zone of lateral roots where auxin is accumulating. However, treatment with the auxin transport inhibitor N-1-naphthylphthalamic acid significantly reduced PHOT1 expression in this zone. In addition, PHOT1 expression was higher in darkness than in light. The total number of lateral roots was higher in the phot1 mutant than in wild-type Arabidopsis. Cells in the elongation zone of lateral roots of the phot1 mutant were longer than those of wild-type lateral roots. These findings suggest that PHOT1 plays a role(s) in elongation of lateral roots through the control of an auxin-related signalling pathway.
我们研究了蓝光受体光受体 1（phot1）与拟南芥幼苗侧根生长之间的关系。荧光和共聚焦显微镜图像以及 PHOT1 mRNA 表达研究表明，它在侧根伸长区高度表达，生长素在该处积累。然而，用生长素运输抑制剂 N-1-萘基邻苯二甲酸处理显著降低了该区域的 PHOT1 表达。此外，PHOT1 的表达在黑暗中高于在光下。光 1 突变体的侧根总数高于野生型拟南芥。光 1 突变体侧根伸长区的细胞比野生型侧根的细胞长。这些发现表明 PHOT1 通过控制生长素相关信号通路在侧根伸长中发挥作用。
