Nima Zeid A, Mahmood Meena, Xu Yang, Mustafa Thikra, Watanabe Fumiya, Nedosekin Dmitry A, Juratli Mazen A, Fahmi Tariq, Galanzha Ekaterina I, Nolan John P, Basnakian Alexei G, Zharov Vladimir P, Biris Alexandru S
Center for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, 2801 S. University Avenue, Little Rock, Arkansas 72204, USA.
Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences, 4301 W. Markham St, Little Rock, Arkansas 72205, USA.
Sci Rep. 2014 May 9;4:4752. doi: 10.1038/srep04752.
Nanotechnology has been extensively explored for cancer diagnostics. However, the specificity of current methods to identify simultaneously several cancer biomarkers is limited due to color overlapping of bio-conjugated nanoparticles. Here, we present a technique to increase both the molecular and spectral specificity of cancer diagnosis by using tunable silver-gold nanorods with narrow surface-enhanced Raman scattering (SERS) and high photothermal contrast. The silver-gold nanorods were functionalized with four Raman-active molecules and four antibodies specific to breast cancer markers and with leukocyte-specific CD45 marker. More than two orders of magnitude of SERS signal enhancement was observed from these hybrid nanosystems compared to conventional gold nanorods. Using an antibody rainbow cocktail, we demonstrated highly specific detection of single breast cancer cells in unprocessed human blood. By integrating multiplex targeting, multicolor coding, and multimodal detection, our approach has the potential to improve multispectral imaging of individual tumor cells in complex biological environments.
纳米技术已被广泛用于癌症诊断。然而，由于生物共轭纳米颗粒的颜色重叠，当前同时识别多种癌症生物标志物的方法的特异性有限。在此，我们提出一种技术，通过使用具有窄表面增强拉曼散射（SERS）和高光热对比度的可调谐银金纳米棒，提高癌症诊断的分子特异性和光谱特异性。银金纳米棒用四种拉曼活性分子、四种针对乳腺癌标志物的抗体以及白细胞特异性CD45标志物进行功能化。与传统金纳米棒相比，从这些混合纳米系统中观察到SERS信号增强了两个多数量级。使用抗体彩虹鸡尾酒，我们展示了在未处理的人血中对单个乳腺癌细胞的高特异性检测。通过整合多重靶向、多色编码和多模态检测，我们的方法有潜力改善复杂生物环境中单个肿瘤细胞的多光谱成像。
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