NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2014 Aug;64(Pt 8):2566-2572. doi: 10.1099/ijs.0.059972-0. Epub 2014 May 8.
We isolated two strains of a novel Lipomyces species from soil collected in Chichibu forest, Saitama prefecture, Japan. Based on their morphological and biochemical characteristics, along with multilocus sequence typing using the D1/D2 domain of the large-subunit (LSU) rRNA gene, the internal transcribed spacer (ITS) region and the translation elongation factor 1 alpha gene (EF-1α), the two strains were shown to represent a novel species of the genus Lipomyces, described as Lipomyces chichibuensis sp. nov. (type strain CB08-2(T) = NBRC 109582(T) = CBS 12929(T); Mycobank no. MB808164). In addition, we reidentified the type strains of Lipomyces kononenkoae and Lipomyces spencermartinsiae maintained in culture collections based on phenotypic characters and/or DNA-DNA hybridization to ensure correct future identification of species of the genus Lipomyces. The correct type strains of L. kononenkoae and L. spencermartinsiae are NBRC 107661(T) ( = CBS 2514(T)) and NBRC 10376(T) ( = CBS 5608(T)), respectively.
我们从日本埼玉县秩父森林采集的土壤中分离到两株新型 Lipomyces 菌株。基于其形态学和生物化学特征，以及使用大亚基（LSU）rRNA 基因的 D1/D2 结构域、内部转录间隔区（ITS）区域和翻译延伸因子 1α 基因（EF-1α）的多位序列分型，这两株菌株被证明代表了 Lipomyces 属的一个新种，命名为 Lipomyces chichibuensis sp. nov.（模式株 CB08-2(T) = NBRC 109582(T) = CBS 12929(T)；Mycobank 编号 MB808164）。此外，我们还根据表型特征和/或 DNA-DNA 杂交重新鉴定了保藏在培养物中的 Lipomyces kononenkoae 和 Lipomyces spencermartinsiae 的模式菌株，以确保正确识别 Lipomyces 属的未来物种。L. kononenkoae 和 L. spencermartinsiae 的正确模式菌株分别为 NBRC 107661(T)（ = CBS 2514(T)）和 NBRC 10376(T)（ = CBS 5608(T)）。
