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日本分离到的 Lipomyces chichibuensis sp. nov.,以及 Lipomyces kononenkoae 和 Lipomyces spencermartinsiae 模式菌株的重新鉴定。

Lipomyces chichibuensis sp. nov., isolated in Japan, and reidentification of the type strains of Lipomyces kononenkoae and Lipomyces spencermartinsiae.

机构信息

NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.

出版信息

Int J Syst Evol Microbiol. 2014 Aug;64(Pt 8):2566-2572. doi: 10.1099/ijs.0.059972-0. Epub 2014 May 8.

Abstract

We isolated two strains of a novel Lipomyces species from soil collected in Chichibu forest, Saitama prefecture, Japan. Based on their morphological and biochemical characteristics, along with multilocus sequence typing using the D1/D2 domain of the large-subunit (LSU) rRNA gene, the internal transcribed spacer (ITS) region and the translation elongation factor 1 alpha gene (EF-1α), the two strains were shown to represent a novel species of the genus Lipomyces, described as Lipomyces chichibuensis sp. nov. (type strain CB08-2(T) = NBRC 109582(T) = CBS 12929(T); Mycobank no. MB808164). In addition, we reidentified the type strains of Lipomyces kononenkoae and Lipomyces spencermartinsiae maintained in culture collections based on phenotypic characters and/or DNA-DNA hybridization to ensure correct future identification of species of the genus Lipomyces. The correct type strains of L. kononenkoae and L. spencermartinsiae are NBRC 107661(T) ( = CBS 2514(T)) and NBRC 10376(T) ( = CBS 5608(T)), respectively.

摘要

我们从日本埼玉县秩父森林采集的土壤中分离到两株新型 Lipomyces 菌株。基于其形态学和生物化学特征,以及使用大亚基(LSU)rRNA 基因的 D1/D2 结构域、内部转录间隔区(ITS)区域和翻译延伸因子 1α 基因(EF-1α)的多位序列分型,这两株菌株被证明代表了 Lipomyces 属的一个新种,命名为 Lipomyces chichibuensis sp. nov.(模式株 CB08-2(T) = NBRC 109582(T) = CBS 12929(T);Mycobank 编号 MB808164)。此外,我们还根据表型特征和/或 DNA-DNA 杂交重新鉴定了保藏在培养物中的 Lipomyces kononenkoae 和 Lipomyces spencermartinsiae 的模式菌株,以确保正确识别 Lipomyces 属的未来物种。L. kononenkoae 和 L. spencermartinsiae 的正确模式菌株分别为 NBRC 107661(T)( = CBS 2514(T))和 NBRC 10376(T)( = CBS 5608(T))。

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