利用 X 射线自由电子激光确定对 X 射线敏感的蛋白质无损晶体结构
Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL.
机构信息
1] RIKEN SPring-8 Center, Sayo, Japan. [2] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [3].
1] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [2].
出版信息
Nat Methods. 2014 Jul;11(7):734-6. doi: 10.1038/nmeth.2962. Epub 2014 May 11.
We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.
我们报告了一种飞秒晶体学方法，用于使用大单晶和 X 射线自由电子激光（XFEL）的飞秒脉冲，在亚埃空间分辨率下解决大蛋白质的无辐射损伤晶体结构。我们通过确定牛细胞色素 c 氧化酶的 1.9 Å 无辐射损伤结构，证明了该方法的性能，牛细胞色素 c 氧化酶是一种大型（420 kDa）、对辐射高度敏感的膜蛋白。
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