Glasgow Jeff, Tullman-Ercek Danielle
Department of Chemistry, University of California Berkeley, Berkeley, CA, 94720, USA.
Appl Microbiol Biotechnol. 2014 Jul;98(13):5847-58. doi: 10.1007/s00253-014-5787-3. Epub 2014 May 10.
As biological agents, viruses come in an astounding range of sizes, with varied shapes and surface morphologies. The structures of viral capsids are generally assemblies of hundreds of copies of one or a few proteins which can be harnessed for use in a wide variety of applications in biotechnology, nanotechnology, and medicine. Despite their complexity, many capsid types form as homogenous populations of precise geometrical assemblies. This is important in both medicine, where well-defined therapeutics are critical for drug performance and federal approval, and nanotechnology, where precise placement affects the properties of the desired material. Here we review the production of viruses and virus-like particles with methods for selecting and manipulating the size, surface chemistry, assembly state, and interior cargo of capsid. We then discuss many of the applications used in research today and the potential commercial and therapeutic products from engineered viral capsids.
作为生物制剂，病毒的大小范围惊人，形状和表面形态各异。病毒衣壳的结构通常是由一种或几种蛋白质的数百个拷贝组装而成，这些蛋白质可用于生物技术、纳米技术和医学等广泛的应用领域。尽管它们很复杂，但许多衣壳类型都是精确几何组装的同质群体。这在医学（明确的治疗方法对药物性能和联邦批准至关重要）和纳米技术（精确放置会影响所需材料的性能）中都很重要。在这里，我们回顾了病毒和病毒样颗粒的生产方法，以及选择和操纵衣壳大小、表面化学、组装状态和内部货物的方法。然后，我们讨论了当今研究中使用的许多应用，以及工程化病毒衣壳潜在的商业和治疗产品。
