Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneungdaehangno, Gangneung, Gangwon 210-702, Republic of Korea.
College of Food Science and Engineering, Northwest A&F University, Yangling, Shanxi 712100, China.
Int J Biol Macromol. 2014 Jul;68:233-40. doi: 10.1016/j.ijbiomac.2014.05.005. Epub 2014 May 10.
Exopolysaccharides (EPS) obtained from the culture medium of Lactobacillus confusus TISTR 1498 were investigated to determine their molecular characteristics and the effect of molecular weight (Mw) on immunomodulatory activity. The EPS mainly consisted of carbohydrates (81.9±2.4%) with only one type of monosaccharide, D-glucose, which was mostly connected by α-(1→6) glycosidic linkages. The EPS itself was unable to stimulate RAW264.7 cells to produce pro-inflammatory mediator nitric oxide (NO) and cytokines. However, considerable stimulation of RAW264.7 cells was observed by the low Mw of EPSs having Mw values≤70×10(3)g/mol. The partially hydrolyzed EPS stimulated RAW264.7 cells to induce considerable NO and various cytokine production such as TNF-α, IL-1β, IL-6 and IL-10 via up-regulation of their mRNA expression. In addition, the degradation Iκ-B and the phosphorylation of c-Jun NH2-terminal kinase (JNK) were facilitated by BW-30 and MW-40, suggesting that the partially hydrolyzed EPS stimulated RAW264.7 cells through the activation of NF-κB and JNK pathways.
从清酒乳杆菌 TISTR1498 培养液中提取的胞外多糖（EPS），对其分子特征以及分子量（Mw）对免疫调节活性的影响进行了研究。EPS 主要由碳水化合物组成（81.9±2.4%），其中仅有一种单糖，即 D-葡萄糖，主要通过α-（1→6）糖苷键连接。EPS 本身不能刺激 RAW264.7 细胞产生促炎介质一氧化氮（NO）和细胞因子。然而，通过 MW 值≤70×10(3)g/mol 的低分子量 EPS 观察到对 RAW264.7 细胞的相当大的刺激。部分水解的 EPS 通过上调其 mRNA 表达，诱导 RAW264.7 细胞产生相当量的 NO 和各种细胞因子，如 TNF-α、IL-1β、IL-6 和 IL-10。此外，BW-30 和 MW-40 促进 Iκ-B 的降解和 c-Jun NH2-末端激酶（JNK）的磷酸化，表明部分水解的 EPS 通过 NF-κB 和 JNK 途径激活 RAW264.7 细胞。
