Oliver Rebekah E, Islamovic Emir, Obert Donald E, Wise Mitchell L, Herrin Lauri L, Hang An, Harrison Stephen A, Ibrahim Amir, Marshall Juliet M, Miclaus Kelci J, Lazo Gerard R, Hu Gongshe, Jackson Eric W
Department of Plant Sciences, North Dakota State University, Fargo, North Dakota, United States of America, formerly USDA-ARS, Small Grains and Potato Germplasm Research Unit, Aberdeen, Idaho, United States of America.
USDA-ARS, Small Grains and Potato Germplasm Research Unit, Aberdeen, Idaho, United States of America.
PLoS One. 2014 May 12;9(5):e96276. doi: 10.1371/journal.pone.0096276. eCollection 2014.
Tocochromanols are recognized for nutritional content, plant stress response, and seed longevity. Here we present a systems biological approach to characterize and develop predictive assays for genes affecting tocochromanol variation in barley. Major QTL, detected in three regions of a SNP linkage map, affected multiple tocochromanol forms. Candidate genes were identified through barley/rice orthology and sequenced in genotypes with disparate tocochromanol profiles. Gene-specific markers, designed based on observed polymorphism, mapped to the originating QTL, increasing R2 values at the respective loci. Polymorphism within promoter regions corresponded to motifs known to influence gene expression. Quantitative PCR analysis revealed a trend of increased expression in tissues grown at cold temperatures. These results demonstrate utility of a novel method for rapid gene identification and characterization, and provide a resource for efficient development of barley lines with improved tocochromanol profiles.
生育三烯酚因营养成分、植物应激反应和种子寿命而受到认可。在此，我们提出一种系统生物学方法，用于表征和开发预测性检测方法，以检测影响大麦生育三烯酚变异的基因。在一个单核苷酸多态性（SNP）连锁图谱的三个区域检测到的主要数量性状基因座（QTL）影响多种生育三烯酚形式。通过大麦/水稻直系同源关系鉴定候选基因，并在具有不同生育三烯酚谱的基因型中进行测序。基于观察到的多态性设计的基因特异性标记映射到原始QTL，增加了各个位点的R2值。启动子区域内的多态性与已知影响基因表达的基序相对应。定量PCR分析显示，在低温下生长的组织中表达有增加的趋势。这些结果证明了一种用于快速基因鉴定和表征的新方法的实用性，并为高效培育具有改良生育三烯酚谱的大麦品系提供了资源。
