Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
J Bras Pneumol. 2014 Mar-Apr;40(2):148-54. doi: 10.1590/s1806-37132014000200008.
To compare the accuracy of the amplified Mycobacterium tuberculosis direct (AMTD) test with reference methods for the laboratory diagnosis of tuberculosis in HIV-infected patients.
This was a study of diagnostic accuracy comparing AMTD test results with those obtained by culture on Löwenstein-Jensen (LJ) medium and by the BACTEC Mycobacteria Growth Indicator Tube 960 (BACTEC MGIT 960) system in respiratory samples analyzed at the Bioassay and Bacteriology Laboratory of the Oswaldo Cruz Foundation Evandro Chagas Clinical Research Institute in the city of Rio de Janeiro, Brazil.
We analyzed respiratory samples collected from 118 patients, of whom 88 (74.4%) were male. The mean age was 36.6 ± 10.6 years. Using the AMTD test, the BACTEC MGIT 960 system, and LJ culture, we identified M. tuberculosis complex in 31.0%, 29.7%, and 27.1% of the samples, respectively. In comparison with LJ culture, the AMTD test had a sensitivity, specificity, positive predictive value, and negative predictive value of 87.5%, 89.4%, 75.7%, and 95.0%, respectively, for LJ culture, whereas, in comparison with the BACTEC MGIT 960 system, it showed values of 88.6%, 92.4%, 83.8%, and 94.8%, respectively.
The AMTD test showed good sensitivity and specificity in the population studied, enabling the laboratory detection of M. tuberculosis complex in paucibacillary respiratory specimens.
比较扩增分枝杆菌直接检测（AMTD）试验与参考方法在 HIV 感染患者结核病实验室诊断中的准确性。
这是一项比较 AMTD 试验结果与在巴西里约热内卢市 Oswaldo Cruz 基金会 Evandro Chagas 临床研究学会生物测定和细菌学实验室分析的呼吸道样本中通过 Löwenstein-Jensen（LJ）培养基培养和 BACTEC 分枝杆菌生长指示剂管 960（BACTEC MGIT 960）系统获得的结果的诊断准确性研究。
我们分析了从 118 例患者收集的呼吸道样本，其中 88 例（74.4%）为男性。平均年龄为 36.6±10.6 岁。使用 AMTD 试验、BACTEC MGIT 960 系统和 LJ 培养，我们分别在 31.0%、29.7%和 27.1%的样本中鉴定出结核分枝杆菌复合群。与 LJ 培养相比，AMTD 试验的灵敏度、特异性、阳性预测值和阴性预测值分别为 87.5%、89.4%、75.7%和 95.0%，而与 BACTEC MGIT 960 系统相比，其值分别为 88.6%、92.4%、83.8%和 94.8%。
在研究人群中，AMTD 试验显示出良好的灵敏度和特异性，能够在少菌呼吸道标本中检测结核分枝杆菌复合群。
