Cancer Genomics Netherlands-Hubrecht Institute-KNAW & University Medical Centre Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
Nat Rev Cancer. 2014 Jun;14(6):406-18. doi: 10.1038/nrc3742.
To comprehend the complexity of cancer, the biological characteristics acquired during the initiation and progression of tumours were classified as the 'hallmarks of cancer'. Intravital microscopy techniques have been developed to study individual cells that acquire these crucial traits, by visualizing tissues with cellular or subcellular resolution in living animals. In this Review, we highlight the latest intravital microscopy techniques that have been used in living animals (predominantly mice) to unravel fundamental and dynamic aspects of various hallmarks of cancer. In addition, we discuss the application of intravital microscopy techniques to cancer therapy, as well as limitations and future perspectives for these techniques.
为了理解癌症的复杂性，在肿瘤的发生和发展过程中获得的生物学特征被归类为“癌症的标志”。活体显微镜技术的发展使得人们可以通过在活体动物中以细胞或亚细胞分辨率可视化组织来研究获得这些关键特征的单个细胞。在这篇综述中，我们强调了活体动物（主要是小鼠）中使用的最新活体显微镜技术，以揭示各种癌症标志的基本和动态方面。此外，我们还讨论了活体显微镜技术在癌症治疗中的应用，以及这些技术的局限性和未来展望。
