青蒿素耐药恶性疟原虫系的表型变化：对休眠和生长抑制敏感性降低的证据。
Phenotypic changes in artemisinin-resistant Plasmodium falciparum lines in vitro: evidence for decreased sensitivity to dormancy and growth inhibition.
机构信息
Drug Resistance and Diagnostics, Australian Army Malaria Institute, Brisbane, Australia.
出版信息
Antimicrob Agents Chemother. 2012 Jan;56(1):428-31. doi: 10.1128/AAC.05456-11. Epub 2011 Oct 10.
Abstract
The appearance of Plasmodium falciparum parasites with decreased in vivo sensitivity but no measurable in vitro resistance to artemisinin has raised the urgent need to characterize the artemisinin resistance phenotype. Changes in the temporary growth arrest (dormancy) profile of parasites may be one aspect of this phenotype. In this study, we investigated the link between dormancy and resistance, using artelinic acid (AL)-resistant parasites. Our results demonstrate that the AL resistance phenotype has (i) decreased sensitivity of mature-stage parasites, (ii) decreased sensitivity of the ring stage to the induction of dormancy, and (iii) a faster recovery from dormancy.
摘要
疟原虫寄生虫外观出现对青蒿素的体内敏感性降低，但体外耐药性无法测量，这就迫切需要对青蒿素耐药表型进行特征描述。寄生虫的临时生长停滞（休眠）状态的变化可能是该表型的一个方面。在这项研究中，我们使用 artelinic 酸（AL）耐药寄生虫来研究休眠与耐药之间的联系。我们的研究结果表明，AL 耐药表型具有：（i）成熟阶段寄生虫的敏感性降低；（ii）环状阶段对休眠诱导的敏感性降低；以及（iii）从休眠中更快地恢复。
相似文献
1
Phenotypic changes in artemisinin-resistant Plasmodium falciparum lines in vitro: evidence for decreased sensitivity to dormancy and growth inhibition.青蒿素耐药恶性疟原虫系的表型变化：对休眠和生长抑制敏感性降低的证据。
Antimicrob Agents Chemother. 2012 Jan;56(1):428-31. doi: 10.1128/AAC.05456-11. Epub 2011 Oct 10.
2
Phenotypic and genotypic analysis of in vitro-selected artemisinin-resistant progeny of Plasmodium falciparum.体外筛选的青蒿素抗性疟原虫后代的表型和基因型分析。
Antimicrob Agents Chemother. 2012 Jan;56(1):302-14. doi: 10.1128/AAC.05540-11. Epub 2011 Nov 14.
3
Plasmodium falciparum founder populations in western Cambodia have reduced artemisinin sensitivity in vitro.柬埔寨西部的恶性疟原虫奠基人群在体外对青蒿素的敏感性有所降低。
Antimicrob Agents Chemother. 2014 Aug;58(8):4935-7. doi: 10.1128/AAC.03055-14. Epub 2014 May 27.
4
Assessment of the induction of dormant ring stages in Plasmodium falciparum parasites by artemisone and artemisone entrapped in Pheroid vesicles in vitro.体外评估青蒿酮及包裹于类脂质体囊泡中的青蒿酮对恶性疟原虫休眠环状体阶段的诱导作用。
Antimicrob Agents Chemother. 2014 Dec;58(12):7579-82. doi: 10.1128/AAC.02707-14. Epub 2014 Oct 6.
5
Decreased Artemisinin Sensitivity of across India.印度出现青蒿素敏感性降低现象。
Antimicrob Agents Chemother. 2019 Sep 23;63(10). doi: 10.1128/AAC.00101-19. Print 2019 Oct.
6
The Spiroindolone KAE609 Does Not Induce Dormant Ring Stages in Plasmodium falciparum Parasites.螺吲哚酮KAE609不会诱导恶性疟原虫寄生虫的休眠环状体。
Antimicrob Agents Chemother. 2016 Aug 22;60(9):5167-74. doi: 10.1128/AAC.02838-15. Print 2016 Sep.
7
Novel phenotypic assays for the detection of artemisinin-resistant Plasmodium falciparum malaria in Cambodia: in-vitro and ex-vivo drug-response studies.柬埔寨青蒿素耐药性恶性疟原虫的新型表型检测方法：体外和体内药物反应研究。
Lancet Infect Dis. 2013 Dec;13(12):1043-9. doi: 10.1016/S1473-3099(13)70252-4. Epub 2013 Sep 11.
8
Fitness of artemisinin-resistant Plasmodium falciparum in vitro.青蒿素耐药恶性疟原虫的体外适应性
J Antimicrob Chemother. 2015 Oct;70(10):2787-96. doi: 10.1093/jac/dkv199. Epub 2015 Jul 22.
9
The artemisinin-induced dormant stages of Plasmodium falciparum exhibit hallmarks of cellular quiescence/senescence and drug resilience.青蒿素诱导的恶性疟原虫休眠阶段表现出细胞静止/衰老和药物耐药的特征。
Nat Commun. 2024 Aug 29;15(1):7485. doi: 10.1038/s41467-024-51846-0.
10
Plasmodium falciparum in vitro continuous culture conditions: A comparison of parasite susceptibility and tolerance to anti-malarial drugs throughout the asexual intra-erythrocytic life cycle.恶性疟原虫体外连续培养条件：在整个红内无性生殖生命周期中对疟原虫敏感性和抗疟药物耐受性的比较。
Int J Parasitol Drugs Drug Resist. 2017 Dec;7(3):295-302. doi: 10.1016/j.ijpddr.2017.07.001. Epub 2017 Jul 15.
引用本文的文献
1
Measuring growth, resistance, and recovery after artemisinin treatment of Plasmodium falciparum in a single semi-high-throughput assay.在单一半高通量试验中测量青蒿素治疗恶性疟原虫后的生长、抗性和恢复情况。
Malar J. 2025 Aug 14;24(1):263. doi: 10.1186/s12936-025-05481-9.
2
Isolation and characterization of blood-stage persisters by improved selection protocols using dihydroartemisinin alone.通过仅使用双氢青蒿素的改进选择方案对血液阶段持续存在的疟原虫进行分离和鉴定。
Antimicrob Agents Chemother. 2025 Mar 5;69(3):e0005324. doi: 10.1128/aac.00053-24. Epub 2025 Feb 10.
3
Measuring Growth, Resistance, and Recovery after Artemisinin Treatment of Plasmodium falciparum in a single semi-high-throughput Assay.在单一半高通量试验中测定青蒿素治疗恶性疟原虫后的生长、抗性及恢复情况。
bioRxiv. 2024 Nov 11:2024.11.11.623064. doi: 10.1101/2024.11.11.623064.
4
The artemisinin-induced dormant stages of Plasmodium falciparum exhibit hallmarks of cellular quiescence/senescence and drug resilience.青蒿素诱导的恶性疟原虫休眠阶段表现出细胞静止/衰老和药物耐药的特征。
Nat Commun. 2024 Aug 29;15(1):7485. doi: 10.1038/s41467-024-51846-0.
5
tRNA modification reprogramming contributes to artemisinin resistance in Plasmodium falciparum.tRNA 修饰重编程有助于疟原虫对青蒿素的耐药性。
Nat Microbiol. 2024 Jun;9(6):1483-1498. doi: 10.1038/s41564-024-01664-3. Epub 2024 Apr 17.
6
MYST regulates DNA repair and forms a NuA4-like complex in the malaria parasite .MYST调节DNA修复并在疟原虫中形成类似NuA4的复合物。
mSphere. 2024 Apr 23;9(4):e0014024. doi: 10.1128/msphere.00140-24. Epub 2024 Apr 2.
7
Unveiling drug-tolerant and persister-like cells in lines derived from patients with cutaneous leishmaniasis.揭示源自皮肤利什曼病患者的细胞系中的耐药和持久样细胞。
Front Cell Infect Microbiol. 2023 Sep 18;13:1253033. doi: 10.3389/fcimb.2023.1253033. eCollection 2023.
8
GCN5 plays a key role in regulating artemisinin resistance-related stress responses.GCN5 在调控青蒿素耐药相关应激反应中发挥关键作用。
Antimicrob Agents Chemother. 2023 Oct 18;67(10):e0057723. doi: 10.1128/aac.00577-23. Epub 2023 Sep 13.
9
A Leak-Free Inducible CRISPRi/a System for Gene Functional Studies in Plasmodium falciparum.一种无渗漏的可诱导 CRISPRi/a 系统，用于疟原虫基因功能研究。
Microbiol Spectr. 2022 Jun 29;10(3):e0278221. doi: 10.1128/spectrum.02782-21. Epub 2022 May 5.
10
Plasmodium falciparum resistance to ACTs: Emergence, mechanisms, and outlook.恶性疟原虫对青蒿素类复方药物的抗药性：出现、机制和展望。
Int J Parasitol Drugs Drug Resist. 2021 Aug;16:102-118. doi: 10.1016/j.ijpddr.2021.05.007. Epub 2021 May 26.
本文引用的文献
1
Artemisinin-induced parasite dormancy: a plausible mechanism for treatment failure.青蒿素诱导的寄生虫休眠：治疗失败的一种合理机制。
Malar J. 2011 Mar 8;10:56. doi: 10.1186/1475-2875-10-56.
2
Intrahost modeling of artemisinin resistance in Plasmodium falciparum.疟原虫青蒿素耐药性的宿主内建模。
Proc Natl Acad Sci U S A. 2011 Jan 4;108(1):397-402. doi: 10.1073/pnas.1006113108. Epub 2010 Dec 20.
3
Artemisinin‐induced dormancy in plasmodium falciparum: duration, recovery rates, and implications in treatment failure.青蒿素诱导恶性疟原虫休眠：持续时间、恢复率及其对治疗失败的影响
J Infect Dis. 2010 Nov 1;202(9):1362-8. doi: 10.1086/656476.
4
Deamplification of pfmdr1-containing amplicon on chromosome 5 in Plasmodium falciparum is associated with reduced resistance to artelinic acid in vitro.恶性疟原虫 5 号染色体上 pfmdr1 基因扩增子的去扩增与体外对 artelinic acid 的耐药性降低有关。
Antimicrob Agents Chemother. 2010 Aug;54(8):3395-401. doi: 10.1128/AAC.01421-09. Epub 2010 Apr 26.
5
Exploring the contribution of candidate genes to artemisinin resistance in Plasmodium falciparum.探讨候选基因对恶性疟原虫青蒿素耐药性的贡献。
Antimicrob Agents Chemother. 2010 Jul;54(7):2886-92. doi: 10.1128/AAC.00032-10. Epub 2010 Apr 26.
6
Role of pfmdr1 amplification and expression in induction of resistance to artemisinin derivatives in Plasmodium falciparum.疟原虫 falciparum 中 pfmdr1 扩增和表达在诱导对青蒿素衍生物耐药中的作用。
Antimicrob Agents Chemother. 2010 Jun;54(6):2455-64. doi: 10.1128/AAC.00947-09. Epub 2010 Mar 29.
7
Increased tolerance to artemisinin in Plasmodium falciparum is mediated by a quiescence mechanism.疟原虫对青蒿素的耐受性增加是由休眠机制介导的。
Antimicrob Agents Chemother. 2010 May;54(5):1872-7. doi: 10.1128/AAC.01636-09. Epub 2010 Feb 16.
8
Artemisinin resistance in Plasmodium falciparum malaria.恶性疟原虫疟疾中的青蒿素耐药性。
N Engl J Med. 2009 Jul 30;361(5):455-67. doi: 10.1056/NEJMoa0808859.
9
Evidence of artemisinin-resistant malaria in western Cambodia.柬埔寨西部出现青蒿素耐药性疟疾的证据。
N Engl J Med. 2008 Dec 11;359(24):2619-20. doi: 10.1056/NEJMc0805011. Epub 2008 Dec 8.
10
Qinghaosu (artemisinin): the price of success.青蒿素：成功的代价
Science. 2008 Apr 18;320(5874):330-4. doi: 10.1126/science.1155165.
Zotero 插件