一种获取富血小板血浆的手工方法。
A manual method to obtain platelet rich plasma.
作者信息
Marques Fabiana Paulino, Ingham Sheila Jean McNeill, Forgas Andrea, Franciozi Carlos Eduardo da Silveira, Sasaki Pedro Henrique, Abdalla Rene Jorge
机构信息
Hospital do Coração , Knee Institute, São Paulo, SP, Brazil, Knee Institute, Hospital do Coração (HCor), São Paulo, SP, Brazil.
Hospital do Coração , Knee Institute, São Paulo, SP, Brazil, Knee Institute, Hospital do Coração (HCor), São Paulo, SP, Brazil ; Universidade Federal de São Paulo, Escola Paulista de Medicina, Department of Orthopedics and Traumatology, São Paulo, SP, Brazil, Department of Orthopedics and Traumatology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
出版信息
Acta Ortop Bras. 2014;22(2):75-7. doi: 10.1590/1413-78522014220200798.
OBJECTIVE
This study is to report a manual method to obtain platelet rich plasma (PRP).
METHODS
For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.
RESULTS
A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.
CONCLUSION
The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor. Level of Evidence IV, Controlled Laboratory Study.
目的
本研究旨在报告一种获取富血小板血浆（PRP）的手工方法。
方法
本研究采集61毫升外周血，以541g离心5分钟。离心将血液分离为三个成分：红细胞、 Buffy 层和富血小板血浆。血液和富血小板血浆样本被送至医院实验室，并对血小板和白细胞进行检测。
结果
对637名献血者的样本进行了评估。血小板产率效率为86.77%，血小板浓度增加因子为2.89倍。白细胞浓度增加因子为1.97倍。
结论
此处描述的方法可产生富含白细胞和血小板的血浆，血小板和白细胞增加因子较高。证据等级IV，对照实验室研究。
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