Zhang Zhaoyuan, Wei Yuxuan, Zhang Changqing, Yuan Ting
Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, P.R.China.
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Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2020 Aug 15;34(8):1025-1030. doi: 10.7507/1002-1892.201911054.
To identify a more popularized preparation protocol of leukocytes-rich platelet-rich plasma (L-PRP) for higher tolerance rate.
The peripheral blood samples of 76 volunteers (45.0 mL/case) were mixed with 5 mL sodium citrate injection for blood transfusion, and L-PRP was prepared by twice centrifugations. All blood samples were divided into three groups according to the parameters of twice centrifugation: experimental group A (12 cases, 400× , 10 minutes for the first time and 1 100× , 10 minutes for the second time), experimental group B (27 cases, 800× , 10 minutes for the first time and 1 100× , 10 minutes for the second time), and control group (37 cases, 1 360× , 10 minutes for the first time and 1 360× , 10 minutes for the second time). The platelet recovery rate and platelet and leukocyte enrichment coefficient of L-PRP in each group were calculated and compared.
After removal of abnormal blood samples (platelet recovery rate was more than 100% or white thrombus), the remaining 55 cases were included in the statistical analysis, including 10 cases in experimental group A, 21 cases in experimental group B, and 24 cases in control group. The platelet enrichment coefficient and platelet recovery rate of experimental group B were significantly higher than those of experimental group A and control group ( <0.05); there was no significant difference between experimental group A and control group ( >0.05). There was no significant difference in leukocyte enrichment coefficient between experimental groups A, B, and control group ( >0.05).
The preparation quality of PRP is affected by various factors, including centrifugal force, centrifugal time, temperature, and operation process, . Twice centrifugation (800× , 10 minutes for the first time and 1 100× , 10 minutes for the second time) is an ideal and feasible centrifugation scheme, which can obtain satisfactory platelet recovery rate and enrichment coefficient with thicker buffy coat, which can reduce the fine operation requirements for operators, improve the fault tolerance rate and generalization.
确定一种更普及的富白细胞富血小板血浆（L-PRP）制备方案，以提高耐受性。
将76名志愿者的外周血样本（45.0 mL/例）与5 mL用于输血的枸橼酸钠注射液混合，通过两次离心制备L-PRP。根据两次离心参数将所有血样分为三组：实验组A（12例，第一次400×，10分钟，第二次1100×，10分钟），实验组B（27例，第一次800×，10分钟，第二次1100×，10分钟），对照组（37例，第一次1360×，10分钟，第二次1360×，10分钟）。计算并比较每组L-PRP的血小板回收率以及血小板和白细胞富集系数。
去除异常血样（血小板回收率超过100%或出现白色血栓）后，将其余55例纳入统计分析，其中实验组A 10例，实验组B 21例，对照组24例。实验组B的血小板富集系数和血小板回收率显著高于实验组A和对照组（P<0.05）；实验组A与对照组之间无显著差异（P>0.05）。实验组A、B和对照组之间的白细胞富集系数无显著差异（P>0.05）。
PRP的制备质量受多种因素影响，包括离心力、离心时间、温度和操作过程等。两次离心（第一次800×，10分钟，第二次1100×，10分钟）是一种理想且可行的离心方案，可获得满意的血小板回收率和富集系数，白膜层更厚，可降低对操作人员精细操作的要求，提高容错率和普及性。
