Department of Life Science, Chung-Ang University, Seoul, 156-756, Republic of Korea.
Department of Biological Sciences, Sungkyunkwan University, Suwon, 440-746, Republic of Korea.
Int J Syst Evol Microbiol. 2014 Aug;64(Pt 8):2897-2901. doi: 10.1099/ijs.0.063669-0. Epub 2014 May 29.
A Gram-stain-positive, strictly aerobic, yellow colony-forming bacterium, designated strain 3-3(T), was isolated from forest soil of Bac Kan Province in Vietnam. Cells were non-motile rods without gliding motility, showing oxidase- and catalase-positive reactions. Growth was observed at 20-37 °C (optimum, 28 °C) and pH 5.5-9.5 (optimum, pH 7.5). The major cellular fatty acids were iso-C(15 : 0), iso-C(15 : 1) G and summed feature 3 (comprising C(16 : 1)ω6c and/or C(16 : 1)ω7c). Strain 3-3(T) contained phosphatidylethanoamine, three unidentified aminolipids and three unidentified lipids. The G+C content of the genomic DNA was 49.5 mol% and the only isoprenoid quinone detected was menaquinone 7 (MK-7). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-3(T) formed a tight phylogenetic lineage with Flavihumibacter petaseus T41(T) with a bootstrap value of 100%. Strain 3-3(T) was related most closely to F. petaseus T41(T) with 97.3% 16S rRNA gene sequence similarity and the level of DNA-DNA relatedness between the two was 9.4±1.2%. Based on phenotypic, chemotaxonomic and molecular features, strain 3-3(T) represents a novel species of the genus Flavihumibacter, for which the name Flavihumibacter solisilvae sp. nov. is proposed. The type strain is 3-3(T) ( = KACC 17917(T) = JCM 19891(T)).
一株革兰氏阳性、严格需氧、黄色菌落形成细菌，被命名为 3-3(T) 菌株，从越南北干省森林土壤中分离得到。细胞为无动力的杆状，无滑行运动，氧化酶和过氧化氢酶呈阳性反应。在 20-37°C（最佳温度 28°C）和 pH5.5-9.5（最佳 pH7.5）下观察到生长。主要细胞脂肪酸为 iso-C(15：0)、iso-C(15：1) G 和总和特征 3（包含 C(16：1)ω6c 和/或 C(16：1)ω7c）。菌株 3-3(T) 含有磷脂酰乙醇胺、三种未鉴定的氨基脂和三种未鉴定的脂类。基因组 DNA 的 G+C 含量为 49.5 mol%，检测到的唯一异戊烯醌是menaquinone 7 (MK-7)。基于 16S rRNA 基因序列的系统发育分析表明，菌株 3-3(T) 与 Flavihumibacter petaseus T41(T) 形成紧密的系统发育谱系，置信度为 100%。菌株 3-3(T) 与 Flavihumibacter petaseus T41(T) 的 16S rRNA 基因序列相似度最高，为 97.3%，两者之间的 DNA-DNA 相关性为 9.4±1.2%。基于表型、化学分类和分子特征，菌株 3-3(T) 代表 Flavihumibacter 属的一个新种，将其命名为 Flavihumibacter solisilvae sp. nov.。模式菌株为 3-3(T)（=KACC 17917(T)=JCM 19891(T)）。
