Department of Microbiology, College of Natural Sciences, Dankook University, Cheonan 330-714, Republic of Korea.
Water Supply & Sewerage Research Division, National Institute of Environmental Research, Incheon 440-170, Republic of Korea.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):3235-3239. doi: 10.1099/ijs.0.063370-0. Epub 2014 Jul 3.
A Gram-reaction-positive, rod-shaped, strictly aerobic and non-motile bacterial strain, designated WS16(T), was isolated from the sediment of a shallow stream located in Cheonan, Korea. The strain grew optimally at 28 °C, at pH 7.0 and in the absence of NaCl. Phylogenetic trees based on 16S rRNA gene sequences suggested that the isolate belonged to the genus Flavihumibacter of the phylum Bacteroidetes. Comparative 16S rRNA gene sequence analysis showed that strain WS16(T) was related most closely to Flavihumibacter petaseus T41(T) (96.8 % similarity). The isolate contained MK-7 as the predominant menaquinone and iso-C15 : 0, iso-C15 : 1 and iso-C17 : 0 3-OH as the major fatty acids. The genomic DNA G+C content of the isolate was 45.9 mol%. The results of a polyphasic taxonomic approach indicated that strain WS16(T) represents a novel species of the genus Flavihumibacter, for which the name Flavihumibacter cheonanensis sp. nov. is proposed. The type strain is WS16(T) ( = KACC 17467(T) = JCM 19322(T)).
一株革兰氏反应阳性、杆状、严格需氧、不运动的细菌菌株，被命名为 WS16(T)，从韩国天安的一条浅溪的沉积物中分离得到。该菌株在 28°C、pH 值为 7.0 和没有 NaCl 的条件下生长最佳。基于 16S rRNA 基因序列的系统发育树表明，该分离株属于拟杆菌门的黄杆菌属。比较 16S rRNA 基因序列分析表明，菌株 WS16(T)与 Flavihumibacter petaseus T41(T)（96.8%相似性）最为密切相关。该分离株含有 MK-7 作为主要的menaquinone，以及 iso-C15:0、iso-C15:1 和 iso-C17:0 3-OH 作为主要的脂肪酸。该分离株的基因组 DNA G+C 含量为 45.9 mol%。多相分类学方法的结果表明，菌株 WS16(T)代表黄杆菌属的一个新种，因此提议将其命名为 Cheonanensis 黄杆菌新种。其模式菌株为 WS16(T)（=KACC 17467(T)=JCM 19322(T)）。
