Seo Ye Lin, Jung Jaejoon, Khan Shehzad Abid, Kim Kyung Hyun, Jeon Che Ok
Department of Life Science, Chung-Ang University, 84, HeukSeok-Ro, Dongjak-Gu, Seoul, 06974, Republic of Korea.
Curr Microbiol. 2020 Oct;77(10):3179-3184. doi: 10.1007/s00284-020-02103-2. Epub 2020 Jun 29.
A novel Gram-stain-positive, strictly aerobic, non-motile, rod-shaped bacterium, designated strain R14T was isolated from steep grove soil and taxonomically characterized using a polyphasic approach. Cells showed oxidase-negative and catalase-positive activities and grew at 25-37 °C (optimum, 30 °C), pH 5.0-8.0 (optimum, pH 6.5), and in the presence of 0-0.5% (w/v) NaCl (optimum, 0%). Phylogenetic trees based on 16S rRNA gene sequences revealed that strain R14T formed a phylogenetic lineage with Flavihumibacter genus members within the family Chitinophagaceae. Comparison of 16S rRNA gene sequences showed that strain R14T shared highest similarities with Flavihumibacter solisilvae 3-3T (97.5%), Flavihumibacter petaseus NBRC 106054T (96.7%), and Flavihumibacter stibioxidans YS-17T (96.1%). The G+C content of strain R14T calculated from its whole genome sequence was 44.6 mol%. Strain R14T contained menaquinone-7 and iso-C, iso-C 3-OH, C, C ω6c, and iso-C-G as the sole respiratory isoprenoid quinone and major cellular fatty acids, respectively. Major polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids, and four unidentified lipids. Based on phylogenetic, phenotypic, and chemotaxonomic characteristics of strain R14T, it could be concluded that strain R14T represents a novel species of the genus Flavihumibacter, for which the name Flavihumibacter soli sp. nov. is proposed. The type strain is R14T (=KACC 21229T = JCM 33473T).
从陡峭的树林土壤中分离出一株新型革兰氏阳性、严格需氧、不运动的杆状细菌，命名为菌株R14T，并采用多相分类法对其进行分类学特征分析。细胞表现出氧化酶阴性和过氧化氢酶阳性活性，在25-37°C（最适温度为30°C）、pH 5.0-8.0（最适pH为6.5）以及0-0.5%（w/v）NaCl（最适浓度为0%）存在的条件下生长。基于16S rRNA基因序列构建的系统发育树表明，菌株R14T与几丁质ophagaceae科的Flavihumibacter属成员形成一个系统发育分支。16S rRNA基因序列比较显示，菌株R14T与土壤黄腐菌Flavihumibacter solisilvae 3-3T（97.5%）、果胶黄腐菌Flavihumibacter petaseus NBRC 106054T（96.7%）和锑氧化黄腐菌Flavihumibacter stibioxidans YS-17T（96.1%）的相似性最高。根据其全基因组序列计算，菌株R14T的G+C含量为44.6 mol%。菌株R14T分别含有甲基萘醌-7和异-C、异-C 3-OH、C、C ω6c以及异-C-G作为唯一的呼吸类异戊二烯醌和主要细胞脂肪酸。主要极性脂类包括磷脂酰乙醇胺、两种未鉴定的氨基脂类和四种未鉴定的脂类。基于菌株R14T的系统发育、表型和化学分类学特征，可以得出结论，菌株R14T代表Flavihumibacter属的一个新物种，为此提出了新名称土壤黄腐菌Flavihumibacter soli sp. nov.。模式菌株为R14T（=KACC 21229T = JCM 33473T）。
