使用基质辅助激光解吸电离飞行时间质谱法鉴定跳蚤种类。
Identification of flea species using MALDI-TOF/MS.
作者信息
Yssouf Amina, Socolovschi Cristina, Leulmi Hamza, Kernif Tahar, Bitam Idir, Audoly Gilles, Almeras Lionel, Raoult Didier, Parola Philippe
机构信息
Aix Marseille Université, Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, CNRS 7278, IRD 198, Inserm 1095, WHO Collaborative Center for Rickettsioses and Other Arthropod-Borne Bacterial Diseases, Faculté de Médecine, 27 bd Jean Moulin, 13385 Marseille cedex 5, France.
Institut Pasteur d'Algérie, 1 Rue du Docteur Laveran, Hamma, Alger, Algeria.
出版信息
Comp Immunol Microbiol Infect Dis. 2014 May;37(3):153-7. doi: 10.1016/j.cimid.2014.05.002. Epub 2014 May 17.
In the present study, a molecular proteomics (MALDI-TOF/MS) approach was used as a tool for identifying flea vectors. We measured the MS spectra from 38 flea specimens of 5 species including Ctenocephalides felis, Ctenocephalides canis, Archaeopsylla erinacei, Xenopsylla cheopis and Stenoponia tripectinata. A blind test performed with 24 specimens from species included in a library spectral database confirmed that MALDI-TOF/MS is an effective tool for discriminating flea species. Although fresh and 70% ethanol-conserved samples subjected to MALDI-TOF/MS in blind tests were correctly classified, only MS spectra of quality from fresh specimens were sufficient for accurate and significant identification. A cluster analysis highlighted that the MALDI Biotyper can be used for studying the phylogeny of fleas.
在本研究中，分子蛋白质组学（基质辅助激光解吸电离飞行时间质谱，MALDI-TOF/MS）方法被用作鉴定跳蚤媒介的工具。我们测量了包括猫栉首蚤、犬栉首蚤、刺猬古蚤、印鼠客蚤和三栉蝠蚤在内的5个物种的38个跳蚤标本的质谱。对来自库光谱数据库中物种的24个标本进行的盲测证实，MALDI-TOF/MS是区分跳蚤物种的有效工具。尽管在盲测中对新鲜和用70%乙醇保存的样本进行MALDI-TOF/MS分析时分类正确，但只有新鲜标本的高质量质谱才足以进行准确且有意义的鉴定。聚类分析突出表明，MALDI Biotyper可用于研究跳蚤的系统发育。
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