Liu Yafang, Chen Bin, Lu Xinyan, Jiang Dandan, Wang Tao, Geng Ling, Zhang Quanfu, Yang Xing
Integrated Laboratory of Pathogenic Biology, College of Preclinical Medicine, Dali University, Dali, China.
School of Public Health, Dali University, Dali, China.
Front Vet Sci. 2023 Jun 16;10:1218488. doi: 10.3389/fvets.2023.1218488. eCollection 2023.
Fleas are one of the most common ectoparasites in warm-blooded mammals and an important vector of zoonotic diseases with serious medical implications. We sequenced the complete mitochondrial genomes of and for the first time using high-throughput sequencing and constructed phylogenetic relationships. We obtained double-stranded circular molecules of lengths 15,875 and 15,785 bp, respectively, consisting of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, and two control regions. AT-skew was negative in both (-0.022) and (-0.231), while GC-skew was positive in both (0.024/0.248), which produced significant differences in codon usage and amino acid composition. Thirteen PCGs encoding 3,617 and 3,711 codons, respectively, isoleucine and phenylalanine were used most frequently. The tRNA genes all form a typical secondary structure. Construction of phylogenetic trees based on Bayesian inference (BI) and maximum likelihood (ML) methods for PCGs. The results of this study provide new information for the mitochondrial genome database of fleas and support further taxonomic studies and population genetics of fleas.
跳蚤是温血哺乳动物中最常见的体外寄生虫之一，也是具有严重医学意义的人畜共患病的重要传播媒介。我们首次使用高通量测序对[两种跳蚤名称未给出]的完整线粒体基因组进行了测序，并构建了系统发育关系。我们分别获得了长度为15,875和15,785 bp的双链环状分子，由13个蛋白质编码基因、22个转运RNA、2个核糖体RNA和两个控制区组成。[两种跳蚤名称未给出]的AT偏斜均为负值（分别为-0.022和-0.231），而GC偏斜均为正值（分别为0.024/0.248），这在密码子使用和氨基酸组成上产生了显著差异。13个蛋白质编码基因分别编码3,617和3,711个密码子，其中异亮氨酸和苯丙氨酸使用最为频繁。转运RNA基因均形成典型的二级结构。基于贝叶斯推断（BI）和最大似然（ML）方法构建了蛋白质编码基因的系统发育树。本研究结果为跳蚤线粒体基因组数据库提供了新信息，并支持进一步的跳蚤分类学研究和群体遗传学研究。
