Sharma Punita, Sharma Swati, Maurya Rakesh Kumar, Das De Tanwee, Thomas Tina, Lata Suman, Singh Namita, Pandey Kailash Chand, Valecha Neena, Dixit Rajnikant
Host-Parasite Interaction Biology Group, National Institute of Malaria Research, Sector-8, Dwarka, Delhi 110077, India.
Parasit Vectors. 2014 May 20;7:235. doi: 10.1186/1756-3305-7-235.
In recent years, it has been well documented that gut flora not only influence mosquito physiology, but also significantly alter vector competency. Although, salivary gland and gut constitute key partners of the digestive system, it is still believed that salivary glands may harbor less flora than gut (Parasit Vectors 6: 146, 2013).
Using a metagenomic approach, we have identified for the first time the diverse microbial community associated with these two physiologically different tissues of the digestive system in the mosquito Anopheles culicifacies.
A total of 17 different phyla could be assigned to the whole metagenomic dataset, predominated by the phylum Proteobacteria, Firmicutes, Bacteriodetes, Tenericutes and Actinomycetes. Common bacteria included the members of Enhydrobacter, Agromonas, Serratia, Ralsonia, Lactobacillus, Pseudomonas, Streptococcus, Rubrobacter, Anaerococcus, Methylobacterium, Turicibacter, Elizabethkingia etc. in both the tissues representing 'core microbiota' of the mosquito digestive system. Salivary associated unique bacterial community included the members of Chloriflexi, Chlorobi, Cyanobacteria, Nitrospira, TM7, Armatimonadetes, Planctomycetes, Fibrobacteres etc.
We find that the salivary gland microbial community structure is more diverse than gut of the mosquito, probably due to differential feeding associated engagements such as food acquisition, ingestion and digestion processes.
近年来，有充分的文献记载表明肠道菌群不仅影响蚊子的生理机能，还会显著改变其传播能力。尽管唾液腺和肠道是消化系统的关键组成部分，但人们仍然认为唾液腺中的菌群可能比肠道中的少（《寄生虫与媒介》6: 146, 2013）。
我们采用宏基因组学方法，首次鉴定了与库蚊属按蚊消化系统这两个生理功能不同的组织相关的多样微生物群落。
整个宏基因组数据集可归为17个不同的门，其中以变形菌门、厚壁菌门、拟杆菌门、柔膜菌门和放线菌门为主。常见细菌包括嗜水生菌属、农业单胞菌属、沙雷氏菌属、罗尔斯通氏菌属、乳杆菌属、假单胞菌属、链球菌属、红杆菌属、厌氧球菌属、甲基杆菌属、Turicibacter、伊丽莎白菌属等，这些细菌存在于代表蚊子消化系统“核心微生物群”的两个组织中。与唾液相关的独特细菌群落包括绿弯菌门、绿菌门、蓝细菌门、硝化螺旋菌门、TM7、装甲菌门、浮霉菌门、纤维杆菌门等的成员。
我们发现蚊子唾液腺的微生物群落结构比肠道更为多样，这可能是由于取食相关活动（如食物获取、摄取和消化过程）的差异所致。
