Hindi Osama Ali, Pinarbasi Begum, Bakici Merve, Demirtas Oya Burcin, Gokyer Seyda, Buyuksungur Arda, Orhan Kaan, Oto Cagdas, Yilgor Pinar
Department of Biomedical Engineering, Ankara University, Ankara 06100, Turkiye.
Department of Surgery, Kirikkale University Faculty of Veterinary Medicine Kirikkale 71450, Turkiye.
ACS Biomater Sci Eng. 2025 Aug 11;11(8):5027-5037. doi: 10.1021/acsbiomaterials.5c00780. Epub 2025 Jul 24.
In situ bioprinting represents an innovative approach in tissue engineering and regenerative medicine, enabling direct deposition of bioinks within the body to create or repair tissues at the target site. This technique leverages advanced bioprinting technologies to deliver cells, biomaterials, and bioactive molecules in a precise, controlled manner, offering the potential for on-demand tissue repair and minimizing the need for extensive surgical intervention. In this research, we apply for the first time in the literature a standard 3D bioprinter to perform in situ bioprinting over the bone defects of live animals under anesthesia and discuss the bone regeneration potential. For this, critical-sized bone defects were created on the parietal bones of the rabbits, followed by the application of autologous adipose-derived stem cell-laden bioink using a 3D bioprinter. Postoperative evaluations included micro-CT and histopathological analysis to assess bone healing and bone-material integration. The results demonstrated successful bone regeneration with the in situ bioprinting approach, as compared to the sham and the use of bioink-only. In conclusion, this study contributes to the growing body of evidence supporting in situ 3D bioprinting as a viable and promising technique for craniofacial bone regeneration, with potential implications for broader clinical relevance and paves the way for future clinical applications.
原位生物打印是组织工程和再生医学中的一种创新方法，能够将生物墨水直接沉积在体内，以在目标部位创建或修复组织。该技术利用先进的生物打印技术，以精确、可控的方式递送细胞、生物材料和生物活性分子，为按需组织修复提供了潜力，并最大限度地减少了广泛手术干预的需求。在本研究中，我们首次在文献中应用标准3D生物打印机在麻醉下对活体动物的骨缺损进行原位生物打印，并探讨骨再生潜力。为此，在兔子的顶骨上制造临界尺寸的骨缺损，然后使用3D生物打印机应用负载自体脂肪来源干细胞的生物墨水。术后评估包括微型计算机断层扫描（micro-CT）和组织病理学分析，以评估骨愈合和骨与材料的整合情况。结果表明，与假手术组和仅使用生物墨水组相比，原位生物打印方法成功实现了骨再生。总之，本研究为越来越多的证据提供了补充，支持原位3D生物打印作为一种可行且有前景的颅面骨再生技术，具有更广泛的临床相关性，并为未来的临床应用铺平了道路。
