Gabr Sami A, Berika Mohamed Y, Alghadir Ahmad H
Department of Anatomy, Faculty of Medicine, Mansoura University, Mansoura, Egypt ; Rehabilitation Research Chair, College of Applied Medical Sciences, King Saud University, Riyad, Saudi Arabia.
Department of Anatomy, Faculty of Medicine, Mansoura University, Mansoura, Egypt ; Rehabilitation Research Chair, College of Applied Medical Sciences, King Saud University, Riyad, Saudi Arabia ; Rehabilitation Science Department, College of Applied Medical Sciences, King Saud University, Riyad, Saudi Arabia ; Medical Experimental Research Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Indian J Dermatol. 2014 May;59(3):230-6. doi: 10.4103/0019-5154.131377.
It has been proposed that hepatitis C virus (HCV) antigens are involved in the pathogenesis of psoriasis and may contribute to severity of the disease. Increased expression of the apoptosis-regulating proteins p53 and tTG and decreased levels of bcl-2 in the keratinocytes of the skin of psoriatic patients have been reported.
This study aims to identify the serum levels of apoptosis-regulating proteins in patients with psoriasis and without HCV infection and to study the relation between clinical severity of psoriasis and the presence of HCV infection.
Disease severity was assessed by psoriasis area severity index score (PASI) of 90 patients with psoriasis grouped as mild (n = 30), moderate (n = 30) and severe (n = 30); 20 healthy individuals were used as controls. All groups were subjected for complete history taking, clinical examination, and tests for liver function and HCV infection. The serum levels of apoptosis related proteins: p53, tTG and bcl-2 were estimated by enzyme linked immune sorbent assay (ELISA).
There was a statistically significant (P < 0.001) correlation between clinical severity of psoriasis and presence of HCV antibodies and HCV-mRNA. In addition, significantly (P < 0.001) raised serum p53 and tTG, and reduced bcl-2 were observed among HCV-positive patients as compared to HCV-negative patients and control patients.
These results conclude that clinical severity of psoriasis is affected by the presence of HCV antibodies and overexpression of apoptotic related proteins. In addition, altered serum levels of apoptosis-regulating proteins could be useful prognostic markers and therapeutic targets of psoriatic disease.
有人提出丙型肝炎病毒（HCV）抗原参与银屑病的发病机制，并可能导致疾病的严重程度增加。据报道，银屑病患者皮肤角质形成细胞中凋亡调节蛋白p53和组织转谷氨酰胺酶（tTG）表达增加，而bcl-2水平降低。
本研究旨在确定未感染HCV的银屑病患者血清中凋亡调节蛋白的水平，并研究银屑病的临床严重程度与HCV感染之间的关系。
通过银屑病面积严重程度指数评分（PASI）评估90例银屑病患者的疾病严重程度，将其分为轻度（n = 30）、中度（n = 30）和重度（n = 30）；20名健康个体作为对照。所有组均进行完整的病史采集、临床检查以及肝功能和HCV感染检测。通过酶联免疫吸附测定（ELISA）法测定血清中凋亡相关蛋白p53、tTG和bcl-2的水平。
银屑病的临床严重程度与HCV抗体和HCV-mRNA的存在之间存在统计学显著相关性（P < 0.001）。此外，与HCV阴性患者和对照患者相比，HCV阳性患者血清p53和tTG显著升高（P < 0.001），而bcl-2降低。
这些结果表明，银屑病的临床严重程度受HCV抗体的存在和凋亡相关蛋白的过表达影响。此外，凋亡调节蛋白血清水平的改变可能是银屑病有用的预后标志物和治疗靶点。
