Microbial Biotechnology and Genomics, CSIR -Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, New Delhi, 110007 India ; Department of Biotechnology, University of Pune, Pune, 411007 India.
TERI University, 10, Industrial Area, Vasant Kunj, New Delhi, 110070 India.
Indian J Microbiol. 2014 Sep;54(3):262-7. doi: 10.1007/s12088-014-0467-7. Epub 2014 Apr 29.
Vegetable wastes (VW) and food wastes (FW) are generated in large quantities by municipal markets, restaurants and hotels. Waste slurries (250 ml) in 300 ml BOD bottles, containing 3, 5 and 7 % total solids (TS) were hydrolyzed with bacterial mixtures composed of: Bacillus, Acinetobacter, Exiguobacterium, Pseudomonas, Stenotrophomonas and Sphingobacterium species. Each of these bacteria had high activities for the hydrolytic enzymes: amylase, protease and lipase. Hydrolysate of biowaste slurries were subjected to defined mixture of H2 producers and culture enriched for methanogens. The impact of hydrolysis of VW and FW was observed as 2.6- and 2.8-fold enhancement in H2 yield, respectively. Direct biomethanation of hydrolysates of VW and FW resulted in 3.0- and 1.15-fold improvement in CH4 yield, respectively. A positive effect of hydrolysis was also observed with biomethanation of effluent of H2 production stage, to the extent of 1.2- and 3.5-fold with FW and VW, respectively. The effective H2 yields were 17 and 85 l/kg TS fed, whereas effective CH4 yields were 61.7 and 63.3 l/kg TS fed, from VW and FW, respectively. This ecobiotechnological strategy can help to improve the conversion efficiency of biowastes to biofuels.
蔬菜废弃物(VW)和食物废弃物(FW)在农贸市场、餐馆和酒店中大量产生。在含有 3%、5%和 7%总固体(TS)的 300ml BOD 瓶中，将 250ml 废物泥浆与细菌混合物水解，细菌混合物由芽孢杆菌、不动杆菌、极端嗜热菌、假单胞菌、寡养单胞菌和鞘氨醇杆菌属组成。这些细菌都具有很高的水解酶活性，如淀粉酶、蛋白酶和脂肪酶。生物废物泥浆的水解物被用于定义的混合氢气产生菌和培养物中富集产甲烷菌。VW 和 FW 的水解作用分别使氢气产量提高了 2.6 倍和 2.8 倍。VW 和 FW 水解物的直接生物甲烷化使 CH4 产量分别提高了 3.0 倍和 1.15 倍。氢气生产阶段的废水进行生物甲烷化也产生了积极的水解效果，FW 和 VW 的效果分别提高了 1.2 倍和 3.5 倍。VW 和 FW 的有效氢气产量分别为 17 和 85l/kg TS，有效 CH4 产量分别为 61.7 和 63.3l/kg TS。这种生态生物技术策略有助于提高生物废物转化为生物燃料的效率。
