Patel Sanjay K S, Lee Jung-Kul, Kalia Vipin C
Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi, 110007 India.
Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 143-701 Korea.
Indian J Microbiol. 2017 Jun;57(2):171-176. doi: 10.1007/s12088-017-0643-7. Epub 2017 Mar 9.
Biological hydrogen (H) production from the biowastes is widely recognized as a suitable alternative approach to utilize low cost feed instead of costly individual sugars. In the present investigation, pure and mixed biowastes were fermented by defined sets of mixed cultures for hydrolysis and H production. Under batch conditions, up to 65, 67 and 70 L H/kg total solids (2%, TS) were evolved from apple pomace (AP), onion peels (OP) and potato peels (PP) using a combination of hydrolytic mixed culture (MHC5) and mixed microbial cultures (MMC4 or MMC6), respectively. Among the different combinations of mixed biowastes including AP, OP, PP and pea-shells, the combination of OP and PP exhibited maximum H production of 73 and 84 L/kg TS with MMC4 and MMC6, respectively. This study suggested that H production can be effectively regulated by using defined sets of mixed cultures for hydrolysis and H production from pure and mixed biowastes as feed even under unsterile conditions.
利用生物废弃物生产生物氢被广泛认为是一种合适的替代方法，它可以利用低成本的原料，而不是昂贵的单糖。在本研究中，通过特定的混合培养物对纯生物废弃物和混合生物废弃物进行发酵，以实现水解和产氢。在分批培养条件下，使用水解混合培养物（MHC5）和混合微生物培养物（MMC4或MMC6）的组合，分别从苹果渣（AP）、洋葱皮（OP）和土豆皮（PP）中产生了高达65、67和70升氢气/千克总固体（2%，TS）。在包括AP、OP、PP和豌豆壳在内的混合生物废弃物的不同组合中，OP和PP的组合分别与MMC4和MMC6一起，表现出最高的产氢量，分别为73和84升氢气/千克TS。该研究表明，即使在非无菌条件下，通过使用特定的混合培养物来对纯生物废弃物和混合生物废弃物进行水解和产氢，也可以有效地调节氢气的产生。
