Rabha Aparna Jyoti, Naglot Ashok, Sharma Gauri Dutta, Gogoi Hemant Kumar, Gupta Vikas Kumar, Shreemali Devendra Dutta, Veer Vijay
Department of Life Science and Bioinformatics, Assam University, Silchar 788011, India.
Defence Research Laboratory (DRDO), Tezpur 784001, Assam, India.
J Genet Eng Biotechnol. 2016 Jun;14(1):181-187. doi: 10.1016/j.jgeb.2015.12.003. Epub 2016 Jan 12.
Thirty isolates of endophytic fungi were isolated from healthy asymptomatic leaves of tea plant () and identified morphologically based on colony morphology, spore shape and size, growth and sporulation rate. Internal transcribed spacer r-DNA sequence analysis supported for molecular identification of all the isolates. Based on morphological and molecular characteristics the isolates were identified as . Variations on colony morphology which included the production of conidial masses, led to divide the isolates into different groups. Variations on spore size, growth rate and sporulation rate were exhibited by all the isolates. With RAPD molecular markers, genetic variations among the thirty isolates were observed. Genetic variations and relatedness among the thirty isolates were analyzed with UPGMA phylogram using NTSYS program. Two major groups were obtained among the thirty isolates. Group I comprised of 16 isolates which included three sub groups (Ia, Ib and Ic) and Group II constituted fourteen isolates and it also had three sub groups (IIa, IIb and IIc). A partial co-relationship among the isolates was established on the basis of morphological and molecular based clustering.
从茶树健康无症状叶片中分离出30株内生真菌，并根据菌落形态、孢子形状和大小、生长和产孢率进行形态学鉴定。内部转录间隔区r-DNA序列分析支持对所有分离株进行分子鉴定。根据形态学和分子特征，这些分离株被鉴定为 。菌落形态的变化，包括分生孢子团的产生，导致分离株被分为不同的组。所有分离株均表现出孢子大小、生长速率和产孢率的变化。利用RAPD分子标记，观察到30株分离株之间的遗传变异。使用NTSYS程序通过UPGMA系统发育树分析了30株分离株之间的遗传变异和相关性。30株分离株分为两个主要组。第一组由16株分离株组成，包括三个亚组（Ia、Ib和Ic），第二组由14株分离株组成，也有三个亚组（IIa、IIb和IIc）。基于形态学和分子聚类，在分离株之间建立了部分相关性。
