Korea Polar Research Institute, KIOST, Incheon, Korea; School of Biological Sciences, Seoul National University, Seoul, Korea.
FEMS Microbiol Ecol. 2014 Aug;89(2):465-75. doi: 10.1111/1574-6941.12362.
The subarctic region is highly responsive and vulnerable to climate change. Understanding the structure of subarctic soil microbial communities is essential for predicting the response of the subarctic soil environment to climate change. To determine the composition of the bacterial community and its relationship with soil properties, we investigated the bacterial community structure and properties of surface soil from the moist acidic tussock tundra in Council, Alaska. We collected 70 soil samples with 25-m intervals between sampling points from 0-10 cm to 10-20 cm depths. The bacterial community was analyzed by pyrosequencing of 16S rRNA genes, and the following soil properties were analyzed: soil moisture content (MC), pH, total carbon (TC), total nitrogen (TN), and inorganic nitrogen (NH4+ and NO3-). The community compositions of the two different depths showed that Alphaproteobacteria decreased with soil depth. Among the soil properties measured, soil pH was the most significant factor correlating with bacterial community in both upper and lower-layer soils. Bacterial community similarity based on jackknifed unweighted unifrac distance showed greater similarity across horizontal layers than through the vertical depth. This study showed that soil depth and pH were the most important soil properties determining bacterial community structure of the subarctic tundra soil in Council, Alaska.
北极地区对气候变化高度敏感且脆弱。了解北极土壤微生物群落的结构对于预测北极土壤环境对气候变化的响应至关重要。为了确定细菌群落的组成及其与土壤特性的关系，我们调查了阿拉斯加议会地区湿润酸性丛生苔原的表层土壤的细菌群落结构和特性。我们从 0-10 cm 到 10-20 cm 深度，以 25 m 的间隔收集了 70 个土壤样本。通过 16S rRNA 基因的焦磷酸测序分析了细菌群落，分析了以下土壤特性：土壤水分含量（MC）、pH 值、总碳（TC）、总氮（TN）和无机氮（NH4+和 NO3-）。两个不同深度的群落组成表明，随着土壤深度的增加，α变形菌减少。在所测量的土壤特性中，土壤 pH 值是与上、下层土壤中细菌群落相关性最显著的因素。基于 Jackknifed 非加权 Unifrac 距离的细菌群落相似性表明，水平层之间的相似性大于垂直深度。本研究表明，土壤深度和 pH 值是决定阿拉斯加议会地区北极苔原生态系统土壤细菌群落结构的最重要土壤特性。
