Simple method for isolation and presumptive identification of Clostridium difficile.

Clostridium difficile can be isolated from stools and presumptively identified by inoculating the stools onto CCCBA and the culture looked at under ultra violet light where the golden-yellow fluorescent colonies show up against a dark background. The colonies are then identified by testing them using the API ZYM system and the results further confirmed by testing the culture for toxin production using the tissue culture technique employing Cl. sordellii antitoxin.