Sharma Shukriti, Markham Philip F, Browning Glenn F
Asia-Pacific Centre for Animal Health, School of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia.
PLoS One. 2014 Jun 4;9(6):e97100. doi: 10.1371/journal.pone.0097100. eCollection 2014.
Mycoplasmas are regarded to be useful models for studying the minimum genetic complement required for independent survival of an organism. Mycoplasma bovis is a globally distributed pathogen causing pneumonia, mastitis, arthritis, otitis media and reproductive tract disease, and genome sequences of three strains, the type strain PG45 and two strains isolated in China, have been published. In this study, several Tn4001 based transposon constructs were generated and used to create a M. bovis PG45 insertional mutant library. Direct genome sequencing of 319 independent insertions detected disruptions in 129 genes in M. bovis, 48 of which had homologues in Mycoplasma mycoides subspecies mycoides SC and 99 of which had homologues in Mycoplasma agalactiae. Sixteen genes found to be essential in previous studies on other mycoplasma species were found to be dispensable. Five of these genes have previously been predicted to be part of the core set of 153 essential genes in mycoplasmas. Thus this study has extended the list of non-essential genes of mycoplasmas from that previously generated by studies in other species.
支原体被认为是研究生物体独立生存所需最小遗传互补的有用模型。牛支原体是一种全球分布的病原体，可引起肺炎、乳腺炎、关节炎、中耳炎和生殖道疾病，并且已发表了三株菌株（模式菌株PG45和两株在中国分离的菌株）的基因组序列。在本研究中，构建了几种基于Tn4001的转座子构建体，并用于创建牛支原体PG45插入突变体文库。对319个独立插入进行直接基因组测序，检测到牛支原体中有129个基因发生破坏，其中48个在丝状支原体丝状亚种SC中有同源物，99个在无乳支原体中有同源物。在先前对其他支原体物种的研究中发现必不可少的16个基因被发现是可有可无的。其中5个基因先前被预测为支原体中153个必需基因核心集的一部分。因此，本研究扩展了支原体非必需基因的列表，超出了先前在其他物种研究中所产生的范围。
