Mycoplasma Department, Animal Health Research Institute, Egypt.
Department of Community Health, National University, San Diego, CA USA.
Acta Trop. 2020 Jul;207:105500. doi: 10.1016/j.actatropica.2020.105500. Epub 2020 Apr 21.
In the scientific literature, a small amount of information is found concerning mycoplasmosis in camel species. A variety of pathogens could be causative agents for pneumonia, but walking pneumonia is mostly caused by Mycoplasma with slow development and mild symptoms. The aim of this study was to identify mycoplasmas from camels (Camelus dromedarius) and extending the arsenal of factors implicated in pathogenicity of M. arginini to shed light on the current knowledge gap. 460 lung samples (pneumonic; n=210 and apparently healthy; n=250) were randomly collected from the one-humped camels (C. domedarius) that have been imported from Sudan and slaughtered at Cairo Slaughterhouse. 48 out of 210 isolates (22.9%) recovered from the pneumonic lungs were recorded as M. arginini. Positive PCR results were obtained for all 48 isolates. On the other hand, infection with the organism was not detected in the apparently healthy lungs. Hemolysis and hydrogen sulphide (HS) production, a compound that has previously not been identified as a virulence factor in M. arginini, was evident in 100% of the isolates. The 48 M. arginini isolates were weak in their ability to form biofilm on polystyrene surfaces. All isolates were 100% susceptible to florfenicol and streptomycin and 100% resistant to ciprofloxacin. Resistance to lincomycin, spiromycin, tylosin, doxacyclin and erythromycin was observed at different frequencies. 13 different combinations of antibiotics representing one to four classes were evident with the Macrolide erythromycin being the most represented. It also should be noted that the ciprofloxacin, doxacyclin, lincomycin, erythromycin combination was the most noted in 21/48 isolates. Surprisingly, none of the virulence genes (vsp, uvrC and gapA) and quinolone resistance genes (parC and gyrA) were detected by PCR.
在科学文献中，有关骆驼种支原体病的信息很少。许多病原体都可能导致肺炎，但游走性肺炎主要由支原体引起，其发展缓慢，症状轻微。本研究的目的是从骆驼（Camelus dromedarius）中鉴定支原体，并扩展与 M. arginini 致病性相关的因素，以阐明当前的知识空白。从从苏丹进口并在开罗屠宰场屠宰的单峰驼（C. domedarius）的随机采集了 460 个肺部样本（肺炎；n=210 和明显健康；n=250）。从肺炎肺中分离出的 210 个分离株中有 48 个（22.9%）被记录为 M. arginini。所有 48 个分离株均获得了阳性 PCR 结果。另一方面，在明显健康的肺中未检测到该生物体的感染。100%的分离株都表现出溶血和硫化氢（HS）的产生，而 HS 以前并未被鉴定为 M. arginini 的毒力因子。48 株 M. arginini 分离株在聚苯乙烯表面形成生物膜的能力较弱。所有分离株对氟苯尼考和链霉素的敏感性为 100%，对环丙沙星的耐药性为 100%。林可霉素、螺旋霉素、泰乐菌素、多西环素和红霉素的耐药性以不同的频率出现。有 13 种不同的抗生素组合，代表一到四类，其中大环内酯类红霉素的代表性最强。还应该注意的是，在 21/48 个分离株中，最常见的是环丙沙星、多西环素、林可霉素、红霉素组合。令人惊讶的是，PCR 未检测到任何毒力基因（vsp、uvrC 和 gapA）和喹诺酮类耐药基因（parC 和 gyrA）。
