Gereli Arel, Akgün Umut, Uslu Serap, Ağır Ismail, Ateş Filiz, Nalbantoğlu Ufuk
Department of Orthopedics and Traumatology, Acıbadem University, Faculty of Medicine, İstanbul, Turkey.
Vocational School of Health Services, Acıbadem University, İstanbul, Turkey.
Acta Orthop Traumatol Turc. 2014;48(3):346-54. doi: 10.3944/AOTT.2014.3162.
The aim of this study was to assess the efficacy of a soluble absorbable silicon compound on healing of the Achilles tendon.
The Achilles tendons of 21 Wistar albino rats were cut and repaired. A 0.01 ml organic silicon solution (silanol) was injected peri/intratendinously into the left leg of all rats and the same dose of saline into the right leg postoperatively. Rats were randomly divided into 3 groups for biomechanical testing on Day 10 (7 rats) and Day 20 (7 rats) and histological and immunohistochemical assessment on Day 20 (7 rats). Fibroblast cell count and diameter, tissue vascularity and blood vessel diameter were evaluated by histomorphometry. Basic fibroblast growth factor (bFGF) immunoreactivity was analyzed with immunohistochemistry on Day 20. Failure load and stiffness of the repaired tendons were measured on Days 10 and 20.
The number of fibroblasts per area, average fibroblast diameter, number of vessels parallel to collagen bundles per area and average vessel diameter were significantly higher in the organic silicon group than in the control group (p<0.05). Strong immunoreactivity of bFGF in the silicon group was detected. Failure load was significantly higher in the silicon group than in the control group on Day 10 (p=0.041). On Day 20, while a difference still existed, this difference was not significant. There was no effect of the silicon injection on stiffness of healing tendons.
Organic silicon appears to have a positive effect on tendon healing and is suitable for further studies on host healing response modification.
本研究旨在评估一种可溶性可吸收硅化合物对跟腱愈合的疗效。
切断并修复21只Wistar白化大鼠的跟腱。术后向所有大鼠的左腿周围/肌腱内注射0.01 ml有机硅溶液（硅醇），向右腿注射相同剂量的生理盐水。大鼠在术后第10天（7只大鼠）和第20天（7只大鼠）被随机分为3组进行生物力学测试，并在第20天（7只大鼠）进行组织学和免疫组织化学评估。通过组织形态计量学评估成纤维细胞计数和直径、组织血管化程度和血管直径。在第20天用免疫组织化学分析碱性成纤维细胞生长因子（bFGF）的免疫反应性。在第10天和第20天测量修复肌腱的破坏载荷和刚度。
有机硅组每单位面积的成纤维细胞数量、平均成纤维细胞直径、每单位面积与胶原束平行的血管数量和平均血管直径均显著高于对照组（p<0.05）。在硅组中检测到bFGF的强免疫反应性。在第10天，硅组的破坏载荷显著高于对照组（p = 0.041）。在第20天，虽然差异仍然存在，但不显著。硅注射对愈合肌腱的刚度没有影响。
有机硅似乎对肌腱愈合有积极作用，适用于进一步研究宿主愈合反应的改变。
