Kiyota Hiroshi, Okuda Yukiko, Ito Michiho, Hirai Masami Yokota, Ikeuchi Masahiko
Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
Department of Life Sciences (Biology), Graduate School of Arts and Science, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
J Biotechnol. 2014 Sep 20;185:1-7. doi: 10.1016/j.jbiotec.2014.05.025. Epub 2014 Jun 4.
Isoprenoids, major secondary metabolites in many organisms, are utilized in various applications. We constructed a model photosynthetic production system for limonene, a volatile isoprenoid, using a unicellular cyanobacterium that expresses the plant limonene synthase. This system produces limonene photosynthetically at a nearly constant rate and that can be efficiently recovered using a gas-stripping method. This production does not affect the growth of the cyanobacteria and is markedly enhanced by overexpression of three enzymes in the intrinsic pathway to provide the precursor of limonene, geranyl pyrophosphate. The photosynthetic production of limonene in our system is more or less sustained from the linear to stationary phase of cyanobacterial growth for up to 1 month.
类异戊二烯是许多生物体中的主要次生代谢产物，被用于各种应用。我们构建了一个用于生产挥发性类异戊二烯柠檬烯的光合生产系统，该系统使用表达植物柠檬烯合酶的单细胞蓝细菌。该系统以近乎恒定的速率光合生产柠檬烯，并且可以使用气提方法有效地回收。这种生产不会影响蓝细菌的生长，并且通过在提供柠檬烯前体香叶基焦磷酸的内在途径中过表达三种酶，产量显著提高。在我们的系统中，从蓝细菌生长的对数期到稳定期，柠檬烯的光合生产或多或少可持续长达1个月。
