Jongedijk Esmer, Cankar Katarina, Buchhaupt Markus, Schrader Jens, Bouwmeester Harro, Beekwilder Jules
Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, Wageningen, 6708, PB, The Netherlands.
Plant Research International, PO Box 16, 6700, AA, Wageningen, The Netherlands.
Appl Microbiol Biotechnol. 2016 Apr;100(7):2927-38. doi: 10.1007/s00253-016-7337-7. Epub 2016 Feb 26.
This mini review describes novel, biotechnology-based, ways of producing the monoterpene limonene. Limonene is applied in relatively highly priced products, such as fragrances, and also has applications with lower value but large production volume, such as biomaterials. Limonene is currently produced as a side product from the citrus juice industry, but the availability and quality are fluctuating and may be insufficient for novel bulk applications. Therefore, complementary microbial production of limonene would be interesting. Since limonene can be derivatized to high-value compounds, microbial platforms also have a great potential beyond just producing limonene. In this review, we discuss the ins and outs of microbial limonene production in comparison with plant-based and chemical production. Achievements and specific challenges for microbial production of limonene are discussed, especially in the light of bulk applications such as biomaterials.
这篇小型综述描述了基于生物技术生产单萜柠檬烯的新方法。柠檬烯应用于价格相对较高的产品，如香料，也应用于价值较低但产量大的产品，如生物材料。目前，柠檬烯是柑橘汁产业的副产品，但可得性和质量波动较大，可能不足以满足新型大规模应用的需求。因此，通过微生物补充生产柠檬烯将是一件有趣的事情。由于柠檬烯可以衍生为高价值化合物，微生物平台除了生产柠檬烯之外还有很大潜力。在这篇综述中，我们将微生物生产柠檬烯与基于植物的生产和化学合成生产进行了比较，并讨论了其中的细节。我们还讨论了微生物生产柠檬烯所取得的成果和面临的具体挑战，特别是考虑到生物材料等大规模应用时。
