Qin Chao, Han Zhijian, Qian Jian, Bao Meiling, Li Pu, Ju Xiaobing, Zhang Shaobo, Zhang Lei, Li Shuang, Cao Qiang, Lu Qiang, Li Jie, Shao Pengfei, Meng Xiaoxin, Zhang Wei, Yin Changjun
Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
PLoS One. 2014 Jun 6;9(6):e99372. doi: 10.1371/journal.pone.0099372. eCollection 2014.
Recent large-scale transcriptome analyses have found large numbers of transcripts, including that of long non-coding RNAs (lncRNAs), which are aberrant in various diseases, especially cancers. However, it is not clear whether lncRNAs are involved specifically in renal cell carcinoma (RCC). We investigated the expression patterns of lncRNAs in five RCC tumor samples (T) relative to those of matched adjacent non-tumor tissues (N) via microarray.
A microarray with 33,045 lncRNA probes and 30,215 mRNA probes was used to identify deregulated lncRNAs in five RCC patients. Furthermore, we confirmed the relative expression levels of AK096725 and ENST00000453068 in 70 paired samples by quantitative reverse transcription polymerase chain reaction (qRT-PCR).
The lncRNA microarray revealed 27,279 lncRNAs in RCC samples, of which 480 were significantly upregulated (P<0.05; T/N>1.5) and 417 were significantly downregulated (P<0.05; N/T>1.5) compared with the matched non-tumor samples. In addition, 19,995 mRNAs were detected, of which 458 were significantly upregulated (P<0.05; T/N>1.5) and 413 were significantly downregulated (P<0.05; N/T>1.5). The expression level changes of AK096725 (P = 0.043) and ENST00000453068 (P<0.001) in 70 paired samples were in accord with the microarray data.
The study uncovered expression patterns of lncRNAs in 5 RCC patients, as well as a number of aberrant lncRNAs and mRNAs in tumor samples compared with the non-tumor tissues. The revelation of an association between AK096725 expression and RCC is especially noteworthy. These findings may help to find new biomarkers in RCC.
近期大规模转录组分析发现了大量转录本，包括长链非编码RNA（lncRNA），其在各种疾病尤其是癌症中表现异常。然而，尚不清楚lncRNA是否特异性参与肾细胞癌（RCC）。我们通过微阵列研究了5例RCC肿瘤样本（T）相对于匹配的相邻非肿瘤组织（N）中lncRNA的表达模式。
使用含有33,045个lncRNA探针和30,215个mRNA探针的微阵列，鉴定5例RCC患者中失调的lncRNA。此外，我们通过定量逆转录聚合酶链反应（qRT-PCR）确认了70对样本中AK096725和ENST00000453068的相对表达水平。
lncRNA微阵列在RCC样本中揭示了27,279个lncRNA，与匹配的非肿瘤样本相比，其中480个显著上调（P<0.05；T/N>1.5），417个显著下调（P<0.05；N/T>1.5）。此外，检测到19,995个mRNA，其中458个显著上调（P<0.05；T/N>1.5），413个显著下调（P<0.05；N/T>1.5）。70对样本中AK096725（P = 0.043）和ENST00000453068（P<0.001）的表达水平变化与微阵列数据一致。
该研究揭示了5例RCC患者中lncRNA的表达模式，以及与非肿瘤组织相比肿瘤样本中一些异常的lncRNA和mRNA。AK096725表达与RCC之间关联的揭示尤其值得注意。这些发现可能有助于在RCC中找到新的生物标志物。
