Department of Materials Science and Engineering, Sharif University of Technology, Azadi Avenue, P.O. Box 11365-9466, Tehran, Iran.
Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Azadi Avenue, P.O. Box 11365-9466, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2014 Aug 1;41:240-8. doi: 10.1016/j.msec.2014.04.036. Epub 2014 Apr 26.
Orthopaedic implant-associated infections are one of the most serious complications in orthopaedic surgery and a major cause of implant failure. In the present work, drug-eluting coatings based on chitosan containing various amounts of vancomycin were prepared by a cathodic electrophoretic deposition process on titanium foils. A three-step release mechanism of the antibiotic from the films in a phosphate-buffered saline solution was noticed. At the early stage, physical encapsulation of the drug in the hydrogel network controlled the release rate. At the late stage, however, in vitro degradation/deattachment of chitosan was responsible for the controlled release. Cytotoxicity evaluation of the drug-eluting coatings via culturing in human osteosarcoma cells (MG-63 osteoblast-like cell line) showed no adverse effect on the biocompatibility. Antibacterial tests against Gram-positive Staphylococcus aureus also demonstrated that the infection risk of titanium foils was significantly reduced due to the antibiotic release. Additionally, in vitro electrochemical corrosion studies by polarization technique revealed that the corrosion current density was significantly lower for the titanium foils with drug-eluting coatings compared to that of uncoated titanium.
骨科植入物相关感染是骨科手术中最严重的并发症之一，也是植入物失败的主要原因。在本工作中，通过阴极电泳沉积工艺在钛箔上制备了载有不同含量万古霉素的壳聚糖载药涂层。在磷酸盐缓冲溶液中，观察到抗生素从薄膜中释放的三步释放机制。在早期，药物在水凝胶网络中的物理包封控制了释放速率。然而，在后期，壳聚糖的体外降解/脱落负责控制释放。通过在人骨肉瘤细胞（MG-63 成骨样细胞系）中培养进行的载药涂层细胞毒性评估表明，其对生物相容性没有不良影响。针对革兰氏阳性金黄色葡萄球菌的抗菌测试也表明，由于抗生素的释放，钛箔的感染风险显著降低。此外，通过极化技术进行的体外电化学腐蚀研究表明，与未涂层的钛相比，载药涂层钛箔的腐蚀电流密度显著降低。
