Aykut Veysel, Celik Ugur, Celik Burcu
Department of Ophthalmology, Gaziosmanpaşa Taksim Training and Research Hospital, Istanbul, Turkey,
Int Ophthalmol. 2015 Jun;35(3):381-5. doi: 10.1007/s10792-014-9959-z. Epub 2014 Jun 8.
The aim of the study was to examine the influence of different antibiotics on amniotic membrane epithelium and to observe the related ultrastructural changes using transmission electron microscope (TEM). Prospective comparative laboratory study. Amniotic membrane samples from a single placenta were obtained using a sterilized method. Tissue samples were placed in either saline or antibiotics-containing (penicillin, streptomycin, neomycin, or amphotericin B) solutions. The viability of the amniotic membrane epithelial cells was then assessed for saline and antibiotics using both light microscope and TEM to investigate morphological changes. The ultrastructural examination of amniotic membrane epithelium held in antibiotics-containing solutions showed damage to the cell membrane, rarefaction, and loss of microvilli. Amniotic membrane from the control group showed intact epithelium, with surface microvilli and junctional complexes between the cells and the basal membrane. The destructive effects of antibiotics on freshly obtained amniotic membrane were examined with both light microscopy and transmission electron microscopy and significant differences in the ultrastructure were observed.
本研究的目的是检测不同抗生素对羊膜上皮的影响，并使用透射电子显微镜（TEM）观察相关的超微结构变化。前瞻性比较实验室研究。采用无菌方法从单个胎盘获取羊膜样本。将组织样本置于生理盐水或含抗生素（青霉素、链霉素、新霉素或两性霉素B）的溶液中。然后使用光学显微镜和TEM评估生理盐水和抗生素处理后羊膜上皮细胞的活力，以研究形态学变化。对置于含抗生素溶液中的羊膜上皮进行超微结构检查，结果显示细胞膜受损、微绒毛稀疏和丢失。对照组的羊膜显示上皮完整，表面有微绒毛，细胞与基底膜之间有连接复合体。通过光学显微镜和透射电子显微镜检查了抗生素对新鲜获取的羊膜的破坏作用，并观察到超微结构存在显著差异。
