Granado-Lorencio F, Lagarda M J, Garcia-López F J, Sánchez-Siles L M, Blanco-Navarro I, Alegría A, Pérez-Sacristán B, Garcia-Llatas G, Donoso-Navarro E, Silvestre-Mardomingo R A, Barberá R
Unidad de Vitaminas, Hospital Universitario Puerta de Hierro-Majadahonda, Madrid, Spain; Servicio de Bioquímica Clínica, Hospital Universitario Puerta de Hierro-Majadahonda, Madrid, Spain.
Nutrition and Food Science Area, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
Nutr Metab Cardiovasc Dis. 2014 Oct;24(10):1090-6. doi: 10.1016/j.numecd.2014.04.013. Epub 2014 May 6.
Post-menopausal women are at higher risk of cardiovascular disease and bone demineralization. Phytosterols (PS) may be used for hypercholesterolemia in some groups and β-cryptoxanthin (β-Cx) displays a unique anabolic effect on bone. Our aim was to assess the changes in cardiovascular and bone turnover markers from the oral intake of β-Cx and PS in post-menopausal women.
A randomized, double-blind, crossover study with β-Cx (0.75 mg/day) and PS (1.5 g/day), single and combined, was performed in 38 postmenopausal women. Diet was supplemented with 1 × 250 mL milk-based fruit drink/day for 4 weeks with a wash-out period of 4-weeks in between. Serum β-Cx and PS were determined by UPLC and CG-FID respectively. Outcome variables included markers of bone turnover and cardiovascular risk. Biological effect was assessed by paired t test and generalized estimating equations analysis that included the previous treatment, the order of intervention and the interactions. The intake of beverages containing β-Cx and PS brought about a significant increase in serum levels of β-Cx, β-sitosterol and campesterol. Intervention caused changes in almost all the markers while the order, previous treatment and the interaction did not reach statistical significance. Only the intake of the beverage containing β-Cx plus PS brought about significant decreases in total cholesterol, c-HDL, c-LDL and bone turnover markers.
β-Cx improves the cholesterol-lowering effect of PS when supplied simultaneously and this combination may also be beneficial in reducing risk of osteoporosis.
ClinicalTrials.gov number NCT01074723.
绝经后女性患心血管疾病和骨质脱矿的风险更高。植物甾醇（PS）可用于某些人群的高胆固醇血症治疗，而β-隐黄质（β-Cx）对骨骼具有独特的合成代谢作用。我们的目的是评估绝经后女性口服β-Cx和PS后心血管及骨转换标志物的变化。
对38名绝经后女性进行了一项随机、双盲、交叉研究，分别给予β-Cx（0.75毫克/天）和PS（1.5克/天），单药及联合用药。饮食中每天补充1×250毫升牛奶基水果饮料，持续4周，期间有4周的洗脱期。分别采用超高效液相色谱法（UPLC）和气相色谱-火焰离子化检测法（CG-FID）测定血清β-Cx和PS。观察指标包括骨转换标志物和心血管风险标志物。通过配对t检验和广义估计方程分析评估生物学效应，该分析包括先前治疗、干预顺序及相互作用。摄入含β-Cx和PS的饮料使血清β-Cx、β-谷甾醇和菜油甾醇水平显著升高。干预导致几乎所有标志物发生变化，而干预顺序、先前治疗及相互作用未达到统计学意义。仅摄入含β-Cx加PS的饮料使总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇和骨转换标志物显著降低。
同时提供β-Cx可增强PS的降胆固醇效果，这种联合用药可能对降低骨质疏松风险也有益。
ClinicalTrials.gov编号NCT01074723。
