Mengual-Chuliá Beatriz, Domenis Lorenzo, Robetto Serena, Bravo Ignacio G
Infections and Cancer Laboratory, Catalan Institute of Oncology (ICO), Barcelona, Spain; Bellvitge Institute of Biomedical Research (IDIBELL), Barcelona, Spain.
Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Aosta, Italy.
Vet Microbiol. 2014 Aug 6;172(1-2):108-19. doi: 10.1016/j.vetmic.2014.05.006. Epub 2014 May 14.
Most amniotes are the hosts of many, distantly related papillomaviruses (PVs). Infection by PVs can be asymptomatic, or lead instead to benign or malignant lesions. However, PVs infecting animals and associated with malignancies are still largely understudied. In the present study, we communicate the complete genome of a novel PV found in a nasal neoplasia of a free-ranging alpine chamois (Rupicapra r. rupicapra) in an Italian national park. Long-PCR and cloning approaches followed for Sanger sequencing were used to identify the first PV found in chamois. The genome of the novel virus - RrupPV1 - of 7256 bp in length, presents the classical PV structure, and lacks the interE2-L2 region that hosts the E5 gene in AlphaPVs and in DeltaPVs. The nucleotide identity percentage of the L1 ORF, places RrupPV1 together with OaPV3 in the same genus. The latter is a PV isolated from a squamous cell carcinoma in sheep in Sardinia. Full-genome phylogenetic reconstructions suggest that these two viruses are sister taxa, and that both of them are very distantly related to any other known PV. Many cetartiodactyl species are infected by non-monophyletic PVs. Our results exemplify further the multiple links between the infection by certain, distantly related PVs and the development of diverse cancers in animals and highlight the need of a systematic search of oncogenic and non-oncogenic animal PVs.
大多数羊膜动物是许多亲缘关系较远的乳头瘤病毒（PVs）的宿主。PVs感染可能无症状，也可能导致良性或恶性病变。然而，感染动物并与恶性肿瘤相关的PVs仍在很大程度上未得到充分研究。在本研究中，我们报告了在意大利国家公园一只自由放养的高山羚羊（Rupicapra r. rupicapra）鼻腔肿瘤中发现的一种新型PV的完整基因组。采用长PCR和克隆方法进行桑格测序，以鉴定在羚羊中发现的首个PV。这种新型病毒——RrupPV1——的基因组长度为7256 bp，具有经典的PV结构，并且缺少在αPVs和δPVs中包含E5基因的E2-L2区域。L1开放阅读框的核苷酸同一性百分比将RrupPV1与OaPV3归为同一属。后者是从撒丁岛一只绵羊的鳞状细胞癌中分离出的一种PV。全基因组系统发育重建表明，这两种病毒是姐妹类群，并且它们与任何其他已知的PV都有非常远的亲缘关系。许多鲸偶蹄目物种被非单系的PVs感染。我们的结果进一步例证了某些亲缘关系较远的PVs感染与动物多种癌症发展之间的多重联系，并强调了系统搜索致癌和非致癌动物PVs的必要性。
