Ikeoka Toshiyuki, Hayashida Naomi, Nakazato Mio, Sekita Takaharu, Murata-Mori Fumi, Ando Takao, Abiru Norio, Yamasaki Hironori, Kudo Takashi, Maeda Takahiro, Kawakami Atsushi, Takamura Noboru
Department of Global Health, Medicine and Welfare, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences.
Tohoku J Exp Med. 2014 Jun;233(2):149-53. doi: 10.1620/tjem.233.149.
Recent genome-wide association studies have identified Tribbles homolog 1 (TRIB1) as one of the candidate genes associated with lipid profiles. TRIB1 is known to interact with MAP kinases, thereby regulating their activities. The single nucleotide polymorphism rs2954029 of TRIB1 is located within an intron and is associated with lipid profiles. The aim of the present study is to investigate the TRIB1 rs2954029 (A>T polymorphism) with conventional predictors of coronary artery diseases such as carotid intima-media thickness (CIMT) and cardio-ankle vascular index (CAVI), and with lipid profiles in general population. This study enrolled 2,581 Japanese adults, 942 men and 1,639 women with a median age of 68 years (range 29 to 94 years), who participated in a screening program for the general population living in Goto City, Nagasaki Prefecture, Japan from 2008 to 2010. For the determination of TRIB1 rs2954029 genotypes, the polymerase chain reaction method was used. The differences in each parameter among the TRIB1 rs2954029 genotypes were evaluated using analysis of covariance. Genotype frequencies of TRIB1 rs2954029 in all participants were 25.5% for AA, 50.4% for AT, and 24.0% for TT. In women, the AA genotype showed significantly higher log triglyceride (TG) concentrations than the AT genotype (P = 0.004) and the AT + TT genotypes (P = 0.004). On the other hand, there were no associations with CIMT and CAVI among the TRIB1 rs2954029 genotypes. In conclusion, the TRIB1 rs2954029 is associated with serum TG concentrations in Japanese community-dwelling women.
最近的全基因组关联研究已将 Tribbles 同源物 1（TRIB1）确定为与血脂水平相关的候选基因之一。已知 TRIB1 与丝裂原活化蛋白激酶相互作用，从而调节其活性。TRIB1 的单核苷酸多态性 rs2954029 位于一个内含子内，与血脂水平相关。本研究的目的是调查 TRIB1 rs2954029（A>T 多态性）与冠状动脉疾病的传统预测指标，如颈动脉内膜中层厚度（CIMT）和心踝血管指数（CAVI），以及与普通人群血脂水平的关系。本研究纳入了 2581 名日本成年人，其中 942 名男性和 1639 名女性，年龄中位数为 68 岁（范围为 29 至 94 岁），他们于 2008 年至 2010 年参加了日本长崎县五岛市普通人群的筛查项目。为了确定 TRIB1 rs2954029 的基因型，使用了聚合酶链反应方法。使用协方差分析评估了 TRIB1 rs2954029 基因型之间各参数的差异。所有参与者中 TRIB1 rs2954029 的基因型频率为：AA 型为 25.5%，AT 型为 50.4%，TT 型为 24.0%。在女性中，AA 基因型的对数甘油三酯（TG）浓度显著高于 AT 基因型（P = 0.004）和 AT + TT 基因型（P = 0.004）。另一方面，TRIB1 rs2954029 基因型与 CIMT 和 CAVI 之间没有关联。总之，TRIB1 rs2954029 与日本社区居住女性的血清 TG 浓度相关。
