Université de La Réunion, INSERM, UMR 1188 Diabète Athérothrombose Thérapies Réunion Océan Indien (DéTROI), 97490 Sainte-Clotilde, La Réunion, France.
Habemus Papam, Food Industry, 97470 Saint-Benoit, La Réunion, France.
Nutrients. 2021 Jan 19;13(1):280. doi: 10.3390/nu13010280.
Atherosclerosis is a hallmark of most cardiovascular diseases. The implication of macrophages in this pathology is widely documented, notably for their contribution to lipid accumulation within the arterial wall, associated with oxidative stress and inflammation processes. In order to prevent or limit the atherosclerosis damage, nutritional approaches and medicinal plant-based therapies need to be considered. In Reunion Island, medicinal plant-based beverages are traditionally used for their antioxidant, lipid-lowering and anti-inflammatory properties. The aim of our study was to assess the protective effects of eight medicinal plant decoctions in an in vitro model of RAW 264.7 murine macrophages exposed to pro-atherogenic conditions (oxidized low-density lipoproteins-ox-LDL- Lipopolysaccharides-LPS). The impact of polyphenol-rich medicinal plant decoctions on cell viability was evaluated by Neutral Red assay. Fluorescent ox-LDL uptake was assessed by flow cytometry and confocal microscopy. Activation of NF-κB was evaluated by quantification of secreted alkaline phosphatase in RAW-Blue™ macrophages. Our results show that medicinal plant decoctions limited the cytotoxicity induced by ox-LDL on macrophages. Flow cytometry analysis in macrophages demonstrated that medicinal plant decoctions from and decreased ox-LDL uptake and accumulation by more than 70%. In addition, medicinal plant decoctions also inhibited NF-κB pathway activation in the presence of pro-inflammatory concentrations of LPS. Our data suggest that medicinal plant decoctions exert protective effects on ox-LDL-induced cytotoxicity and limited macrophage lipid uptake. Moreover, herbal preparations displayed anti-inflammatory properties on macrophages that can be of interest for limiting the atherosclerotic process.
动脉粥样硬化是大多数心血管疾病的特征。巨噬细胞在这一病理过程中的作用已被广泛记录，尤其是它们对动脉壁内脂质积累的贡献，这与氧化应激和炎症过程有关。为了预防或限制动脉粥样硬化损伤，需要考虑营养方法和药用植物疗法。在留尼汪岛，药用植物饮料因其具有抗氧化、降血脂和抗炎特性而被传统使用。我们的研究目的是评估八种药用植物汤剂在体外 RAW 264.7 鼠巨噬细胞模型中的保护作用，该模型暴露于致动脉粥样硬化条件下（氧化低密度脂蛋白-ox-LDL-脂多糖-LPS）。通过中性红测定法评估多酚丰富的药用植物汤剂对细胞活力的影响。通过流式细胞术和共聚焦显微镜评估荧光 ox-LDL 的摄取。通过 RAW-Blue™巨噬细胞中碱性磷酸酶分泌的定量评估 NF-κB 的激活。我们的结果表明，药用植物汤剂限制了 ox-LDL 对巨噬细胞的细胞毒性。巨噬细胞中的流式细胞术分析表明， 和 的药用植物汤剂降低了 ox-LDL 的摄取和积累超过 70%。此外，药用植物汤剂还抑制了 NF-κB 通路在促炎浓度 LPS 存在下的激活。我们的数据表明，药用植物汤剂对 ox-LDL 诱导的细胞毒性和巨噬细胞脂质摄取具有保护作用。此外，草药制剂对巨噬细胞具有抗炎特性，这可能有助于限制动脉粥样硬化过程。
