Mendez Derek, Lane Thomas J, Sung Jongmin, Sellberg Jonas, Levard Clément, Watkins Herschel, Cohen Aina E, Soltis Michael, Sutton Shirley, Spudich James, Pande Vijay, Ratner Daniel, Doniach Sebastian
Department of Applied Physics, Menlo Park, CA 94025, USA.
Department of Chemistry, Menlo Park, CA 94025, USA.
Philos Trans R Soc Lond B Biol Sci. 2014 Jul 17;369(1647):20130315. doi: 10.1098/rstb.2013.0315.
Tools to study disordered systems with local structural order, such as proteins in solution, remain limited. Such understanding is essential for e.g. rational drug design. Correlated X-ray scattering (CXS) has recently attracted new interest as a way to leverage next-generation light sources to study such disordered matter. The CXS experiment measures angular correlations of the intensity caused by the scattering of X-rays from an ensemble of identical particles, with disordered orientation and position. Averaging over 15 496 snapshot images obtained by exposing a sample of silver nanoparticles in solution to a micro-focused synchrotron radiation beam, we report on experimental efforts to obtain CXS signal from an ensemble in three dimensions. A correlation function was measured at wide angles corresponding to atomic resolution that matches theoretical predictions. These preliminary results suggest that other CXS experiments on disordered ensembles--such as proteins in solution--may be feasible in the future.
用于研究具有局部结构有序性的无序系统（如溶液中的蛋白质）的工具仍然有限。这种理解对于例如合理的药物设计至关重要。相关X射线散射（CXS）作为一种利用下一代光源研究此类无序物质的方法，最近引起了新的关注。CXS实验测量由来自相同粒子集合体的X射线散射引起的强度的角相关性，这些粒子具有无序的取向和位置。通过将溶液中的银纳米颗粒样品暴露于微聚焦同步辐射束获得15496张快照图像并进行平均，我们报告了在三维空间中从集合体获得CXS信号的实验工作。在对应于原子分辨率的广角处测量了相关函数，其与理论预测相符。这些初步结果表明，未来对无序集合体（如溶液中的蛋白质）进行其他CXS实验可能是可行的。
