Division of Plant Microbe Interactions, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, India.
CSIR-Indian Institute of Toxicology Research, 80, Mahatma Gandhi Marg, Qaiserbagh, Lucknow 226001, India.
Bioresour Technol. 2014 Aug;166:235-42. doi: 10.1016/j.biortech.2014.04.085. Epub 2014 May 9.
The aim of this work was to synthesize gold nanoparticles by Trichoderma viride and Hypocrea lixii. The biosynthesis of the nanoparticles was very rapid and took 10 min at 30 °C when cell-free extract of the T. viride was used, which was similar by H. lixii but at 100 °C. Biomolecules present in cell free extracts of both fungi were capable to synthesize and stabilize the formed particles. Synthesis procedure was very quick and environment friendly which did not require subsequent processing. The biosynthesized nanoparticles served as an efficient biocatalyst which reduced 4-nitrophenol to 4-aminophenol in the presence of NaBH₄ and had antimicrobial activity against pathogenic bacteria. To the best of our knowledge, this is the first report of such rapid biosynthesis of gold nanoparticles within 10 min by Trichoderma having plant growth promoting and plant pathogen control abilities, which served both, as an efficient biocatalyst, and a potent antimicrobial agent.
本工作旨在通过绿色木霉（Trichoderma viride）和栓菌（Hypocrea lixii）合成金纳米粒子。当使用绿色木霉的无细胞提取物时，纳米粒子的生物合成非常迅速，在 30°C 下只需 10 分钟，而栓菌则需要在 100°C 下进行。两种真菌的无细胞提取物中存在的生物分子能够合成和稳定形成的颗粒。合成过程非常迅速且环保，不需要后续处理。生物合成的纳米粒子可用作高效的生物催化剂，在 NaBH₄的存在下将 4-硝基苯酚还原为 4-氨基酚，并对致病菌具有抗菌活性。据我们所知，这是首次在 10 分钟内通过具有植物生长促进和植物病原体控制能力的绿色木霉快速合成金纳米粒子的报道，它既可用作高效的生物催化剂，又可用作有效的抗菌剂。
