Sauder Candice A M, Koziel Jillian E, Choi MiRan, Fox Melanie J, Grimes Brenda R, Badve Sunil, Blosser Rachel J, Radovich Milan, Lam Christina C, Vaughan Melville B, Herbert Brittney-Shea, Clare Susan E
Department of Surgery, Feinberg School of Medicine, Northwestern University, 303 E, Superior Street, Chicago, IL 60611, USA.
BMC Cell Biol. 2014 Jun 10;15:20. doi: 10.1186/1471-2121-15-20.
Normal, healthy human breast tissue from a variety of volunteer donors has become available for research thanks to the establishment of the Susan G. Komen for the Cure® Tissue Bank at the IU Simon Cancer Center (KTB). Multiple epithelial (K-HME) and stromal cells (K-HMS) were established from the donated tissue. Explant culture was utilized to isolate the cells from pieces of breast tissue. Selective media and trypsinization were employed to select either epithelial cells or stromal cells. The primary, non-transformed epithelial cells, the focus of this study, were characterized by immunohistochemistry, flow cytometry, and in vitro cell culture.
All of the primary, non-transformed epithelial cells tested have the ability to differentiate in vitro into a variety of cell types when plated in or on biologic matrices. Cells identified include stratified squamous epithelial, osteoclasts, chondrocytes, adipocytes, neural progenitors/neurons, immature muscle and melanocytes. The cells also express markers of embryonic stem cells.
The cell culture conditions employed select an epithelial cell that is pluri/multipotent. The plasticity of the epithelial cells developed mimics that seen in metaplastic carcinoma of the breast (MCB), a subtype of triple negative breast cancer; and may provide clues to the origin of this particularly aggressive type of breast cancer. The KTB is a unique biorepository, and the normal breast epithelial cells isolated from donated tissue have significant potential as new research tools.
由于印第安纳大学西蒙癌症中心的苏珊·G·科门乳腺癌防治基金会组织库（KTB）的建立，来自众多志愿者捐赠者的正常、健康人体乳腺组织已可用于研究。从捐赠组织中分离出了多种上皮细胞（K-HME）和基质细胞（K-HMS）。采用外植体培养法从乳腺组织块中分离细胞。利用选择性培养基和胰蛋白酶消化法来选择上皮细胞或基质细胞。本研究的重点是原代、未转化的上皮细胞，通过免疫组织化学、流式细胞术和体外细胞培养对其进行了表征。
所有测试的原代、未转化上皮细胞在接种于生物基质中或其表面时，都具有在体外分化为多种细胞类型的能力。鉴定出的细胞包括复层鳞状上皮细胞、破骨细胞、软骨细胞、脂肪细胞、神经祖细胞/神经元、未成熟肌肉细胞和黑素细胞。这些细胞还表达胚胎干细胞标志物。
所采用的细胞培养条件筛选出了一种具有多能性的上皮细胞。所培养的上皮细胞的可塑性类似于三阴性乳腺癌的一种亚型——乳腺化生性癌（MCB）中所见的情况；并且可能为这种特别侵袭性的乳腺癌的起源提供线索。KTB是一个独特的生物样本库，从捐赠组织中分离出的正常乳腺上皮细胞作为新的研究工具具有巨大潜力。
