Ee Robson, Lim Yan-Lue, Kin Lin-Xin, Yin Wai-Fong, Chan Kok-Gan
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.
Sensors (Basel). 2014 Jun 10;14(6):10177-86. doi: 10.3390/s140610177.
Strain RB38 was recovered from a former dumping area in Malaysia. MALDI-TOF mass spectrometry and genomic analysis identified strain RB-38 as Pandoraea pnomenusa. Various biosensors confirmed its quorum sensing properties. High resolution triple quadrupole liquid chromatography-mass spectrometry analysis was subsequently used to characterize the N-acyl homoserine lactone production profile of P. pnomenusa strain RB38, which validated that this isolate produced N-octanoyl homoserine lactone as a quorum sensing molecule. This is the first report of the production of N-octanoyl homoserine lactone by P. pnomenusa strain RB38.
菌株RB38是从马来西亚一个以前的垃圾倾倒场分离得到的。基质辅助激光解吸电离飞行时间质谱(MALDI-TOF)和基因组分析鉴定菌株RB-38为嗜麦芽窄食单胞菌。各种生物传感器证实了其群体感应特性。随后采用高分辨率三重四极杆液相色谱-质谱联用分析来表征嗜麦芽窄食单胞菌RB38菌株的N-酰基高丝氨酸内酯产生情况,验证了该分离株产生N-辛酰高丝氨酸内酯作为群体感应分子。这是关于嗜麦芽窄食单胞菌RB38菌株产生N-辛酰高丝氨酸内酯的首次报道。