College of Environment and Chemical Engineering, Shanghai University, 200444, Shanghai, China.
Environ Sci Pollut Res Int. 2014;21(13):8198-205. doi: 10.1007/s11356-014-2807-x. Epub 2014 Mar 29.
The endophytic bacterium isolated from Scirpus triqueter was proved to be an oil-degraded bacterium. A pot experiment was conducted to investigate the removal ratio of diesel under the combined effect of oil-degraded microorganism (Pseudomonas sp. J4AJ) and S. triqueter. The effect of diesel on plant growth parameters, soil enzymes and microbial community was assessed after 60 days. The results showed that the soils which were planted with S. triqueter and inoculated with J4AJ displayed the highest removal ratio (54.51 ± 0.15%) after 60-day experiment. However, the removal ratio of J4AJ-treated soils was 38.97 ± 0.55%. Diesel was toxic to S. triqueter, as evidenced by growth inhibition during the experimental period. However, the plant height and stem biomass in the soils inoculated with J4AJ significantly increased. The combined effect of S. triqueter and J4AJ improved the enzyme activities of the catalase and dehydrogenase in the contaminated soil. The diversity index in soils under the effect of S. triqueter combined with J4AJ was lower than that of the other soil samples. The principal analysis of phospholipid fatty acid signatures revealed that the combined effect of S. triqueter and J4AJ increased the differences of soil microbial community structure with the other treatments.
从香蒲中分离出的内生细菌被证实是一种可降解石油的细菌。通过盆栽实验，研究了在可降解微生物（假单胞菌 J4AJ）和香蒲联合作用下，柴油的去除率。60 天后，评估了柴油对植物生长参数、土壤酶和微生物群落的影响。结果表明，种植香蒲并接种 J4AJ 的土壤在 60 天实验后显示出最高的去除率（54.51±0.15%）。然而，J4AJ 处理土壤的去除率为 38.97±0.55%。柴油对香蒲有毒，这可以从实验期间的生长抑制得到证明。然而，接种 J4AJ 的土壤中的植物高度和茎生物量显著增加。香蒲和 J4AJ 的联合作用提高了污染土壤中过氧化氢酶和脱氢酶的酶活性。在香蒲与 J4AJ 共同作用下的土壤中，多样性指数低于其他土壤样本。磷脂脂肪酸特征的主成分分析表明，香蒲和 J4AJ 的联合作用增加了土壤微生物群落结构与其他处理的差异。
