Mas Núria, Galiana Irene, Hurtado Silvia, Mondragón Laura, Bernardos Andrea, Sancenón Félix, Marcos María D, Amorós Pedro, Abril-Utrillas Nuria, Martínez-Máñez Ramón, Murguía José Ramón
Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Centro Mixto Universidad Politécnica de Valencia, Universidad de Valencia, Valencia, Spain ; Departamento de Química, Universidad Politécnica de Valencia, Valencia, Spain ; CIBER de Bioingeniería, Biomateriales y Nanomedicina, Madrid, Spain.
CIBER de Bioingeniería, Biomateriales y Nanomedicina, Madrid, Spain.
Int J Nanomedicine. 2014 May 23;9:2597-606. doi: 10.2147/IJN.S59654. eCollection 2014.
pH-sensitive gated mesoporous silica nanoparticles have been synthesized. Increased extracellular pH and internalization into living yeast cells triggered molecular gate aperture and cargo release. Proper performance of the system was demonstrated with nanodevices loaded with fluorescein or with the antifungal agent tebuconazole. Interestingly, nanodevices loaded with tebuconazole significantly enhanced tebuconazole cytotoxicity. As alterations of acidic external pH are a key parameter in the onset of fungal vaginitis, this nanodevice could improve the treatment for vaginal mycoses.
已合成了对pH敏感的门控介孔二氧化硅纳米颗粒。细胞外pH值升高以及其内化进入活酵母细胞会触发分子门孔径的变化和货物释放。装载有荧光素或抗真菌剂戊唑醇的纳米装置证明了该系统的良好性能。有趣的是，装载有戊唑醇的纳米装置显著增强了戊唑醇的细胞毒性。由于酸性外部pH值的改变是霉菌性阴道炎发病的关键参数，这种纳米装置可以改善阴道霉菌病的治疗。
