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从人骨髓中分离出一个IgH基因环状DNA克隆。

Isolation of an IgH gene circular DNA clone from human bone marrow.

作者信息

Abe M, Shiku H

机构信息

Department of Oncology, Nagasaki University School of Medicine, Japan.

出版信息

Nucleic Acids Res. 1989 Jan 11;17(1):163-70. doi: 10.1093/nar/17.1.163.

DOI:10.1093/nar/17.1.163
PMID:2492093
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331542/
Abstract

Circular DNA was obtained from human bone marrow. Then a phage library was prepared and screened by use of two probes of the IgH gene; 5'-DHQ52, containing the 5' flanking region of DHQ52, and JH4.3, containing the sequence from JH3 to the 3' flanking region of JH6. One clone, HBMC-1, that was DHQ52+JH4.3- was obtained. HBMC-1 had the germline IgH region upstream of JH1 and the 3' flanking region of DXP1. A recombination signal sequence flanking the 5' side of the JH1 segment was attached to the recombination signal sequence flanking the 3' side of DXP1 forming a head-to-head structure of two 7mers with 10 nucleotides in-between. HBMC-1 is thus considered to be a circular DNA deleted as a consequence of DXP1-JH1 joining of the IgH gene.

摘要

环状DNA取自人骨髓。然后制备噬菌体文库，并使用IgH基因的两种探针进行筛选；5'-DHQ52，包含DHQ52的5'侧翼区域，以及JH4.3，包含从JH3到JH6的3'侧翼区域的序列。获得了一个克隆HBMC-1，它是DHQ52+JH4.3-。HBMC-1在JH1上游具有种系IgH区域和DXP1的3'侧翼区域。JH1片段5'侧的重组信号序列与DXP1 3'侧的重组信号序列相连，形成两个7聚体的头对头结构，中间有10个核苷酸。因此，HBMC-1被认为是由于IgH基因的DXP1-JH1连接而缺失的环状DNA。

相似文献

1
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引用本文的文献

1
Nucleotide sequence of an immunoglobulin D-J gene fusion from a B-cell prolymphocytic leukaemia.来自B细胞幼淋巴细胞白血病的免疫球蛋白D-J基因融合体的核苷酸序列。
Nucleic Acids Res. 1989 Jun 26;17(12):4879. doi: 10.1093/nar/17.12.4879.

本文引用的文献

1
Human immunoglobulin D segments encoded in tandem multigenic families.串联多基因家族中编码的人类免疫球蛋白D区段。
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Determination of nucleotide sequences in DNA.DNA中核苷酸序列的测定。
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Isolation of an excision product of T-cell receptor alpha-chain gene rearrangements.T细胞受体α链基因重排切除产物的分离
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