Deev S M, Combriato G, Klobeck H G, Zachau H G
Nucleic Acids Res. 1987 Jan 12;15(1):1-14. doi: 10.1093/nar/15.1.1.
The recombination process that joins a VK to a JK segment of an immunoglobulin gene generates a second, reciprocal recombination product called f fragment. In this second product the regions flanking the VK and JK segments in the germline are joined in a head to head fashion. We now analysed f fragments in the human lymphoid cell lines Daudi, JI and IARC/BL41. All three f fragments contain JK1 flanks; the VK derived moiety of f Daudi and f41 could be traced back to known germline VK genes. There is a precise head to head joining of the heptanucleotide signal sequences in f Daudi and fJI while in f41 six nucleotides are present between the signal sequences. In contrast to the VK-JK recombination products, the f fragments were found to lack somatic mutations. The structures of the f fragments are discussed in the context of the VK-JK rearrangement mechanism.
将免疫球蛋白基因的一个VK片段与一个JK片段连接起来的重组过程会产生另一种相互对应的重组产物，称为f片段。在这第二种产物中，种系中VK和JK片段两侧的区域以头对头的方式连接在一起。我们现在分析了人类淋巴母细胞系Daudi、JI和IARC/BL41中的f片段。所有这三个f片段都包含JK1侧翼；f Daudi和f41的VK衍生部分可以追溯到已知的种系VK基因。f Daudi和fJI中的七核苷酸信号序列存在精确的头对头连接，而在f41中，信号序列之间有六个核苷酸。与VK-JK重组产物不同，f片段缺乏体细胞突变。我们在VK-JK重排机制的背景下讨论了f片段的结构。
